Sun In-Cheol, Na Jin Hee, Jeong Seo Young, Kim Dong-Eog, Kwon Ick Chan, Choi Kuiwon, Ahn Cheol-Hee, Kim Kwangmeyung
Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, South Korea.
Pharm Res. 2014 Jun;31(6):1418-25. doi: 10.1007/s11095-013-1142-0. Epub 2013 Aug 9.
The application of gold nanoparticles (AuNPs) in biomedical field was limited due to the low stability in the biological condition. Herein, to enhance stability and tumor targeting ability of AuNPs, their surface was modified with biocompatible glycol chitosan (GC) and the in vivo biodistribution of GC coated AuNPs (GC-AuNPs) were studied through computed tomography (CT).
Polymer-coated gold nanoparticles were produced using GC as a reducing agent and a stabilizer. Their feasibility in biomedical application was explored through CT in tumor-bearing mice.
Stability of gold nanoparticles increased in the physiological condition due to the GC coating layer on the surface. Tomographic images of tumor were successfully obtained in the tumor-xenografted animal model when the GC-AuNPs were used as a CT contrast agent. The tumor targeting property of the gold nanoparticles was due to the properties of GC because GC-AuNPs were accumulated in the tumor, while most of heparin-coated nanoparticles were found in the liver and spleen.
The polymer properties on the surface played an important role in the behavior of gold nanoparticles in the biological condition and the enhanced stability and tumor targeting property of nanoparticles were inherited from GC on the surface.
由于金纳米颗粒（AuNPs）在生物条件下稳定性较低，其在生物医学领域的应用受到限制。在此，为提高AuNPs的稳定性和肿瘤靶向能力，用生物相容性的壳聚糖二醇（GC）对其表面进行修饰，并通过计算机断层扫描（CT）研究了GC包覆的AuNPs（GC-AuNPs）在体内的生物分布。
以GC作为还原剂和稳定剂制备聚合物包覆的金纳米颗粒。通过CT在荷瘤小鼠中探索其在生物医学应用中的可行性。
由于表面的GC涂层，金纳米颗粒在生理条件下的稳定性增加。当将GC-AuNPs用作CT造影剂时，在肿瘤异种移植动物模型中成功获得了肿瘤的断层图像。金纳米颗粒的肿瘤靶向特性归因于GC的性质，因为GC-AuNPs在肿瘤中积累，而大多数肝素包覆的纳米颗粒则在肝脏和脾脏中被发现。
表面的聚合物性质在生物条件下金纳米颗粒的行为中起重要作用，纳米颗粒增强的稳定性和肿瘤靶向特性源自表面的GC。
