Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Bioresour Technol. 2013 Oct;146:208-214. doi: 10.1016/j.biortech.2013.07.008. Epub 2013 Jul 10.
Furfural residues (FR), the abundant lignocellulosic residues from commercial furfural production, were delignified with alkaline peroxide process and then taken as substrates for ethanol production by simultaneous saccharification and fermentation (SSF). It was apparent that the delignification efficiency was increased with higher chemical addition and temperature, reaching the maximum removal (73.5%) of lignin. The widespread accessible-cellulose in FR favored the enzymatic hydrolysis and achieved the considerable bioconversion (75.7% with 5 FPU+10 IU/g substrate). The delignification process increased the relative glucose content and then the bioconversion efficiency, closely relating to the increased specific surface area. As the cellulose contents were higher than 60%, the final conversions conversely fell to around 75%, probably due to the insufficient utilization of all active cellulose with low enzyme cocktails addition. Although the SSF bioconversion slightly decreased as the elevated amount of fermentable cellulose, the maximum of ethanol concentration (16.9 g/L) was expectedly obtained.
糠醛渣(FR)是商业糠醛生产中丰富的木质纤维素残渣,用过氧化氢碱性处理进行脱木质素,然后作为同时糖化和发酵(SSF)生产乙醇的底物。显然,脱木质素效率随着化学添加剂和温度的升高而增加,木质素的最大去除率(73.5%)。FR 中广泛存在的易接触纤维素有利于酶解,并实现了可观的生物转化(5 FPU+10 IU/g 底物时达到 75.7%)。脱木质素过程增加了相对葡萄糖含量,从而提高了生物转化效率,这与比表面积的增加密切相关。当纤维素含量高于 60%时,最终转化率反而下降到 75%左右,可能是由于添加的酶混合物较少,所有活性纤维素的利用率不足。尽管 SSF 生物转化随着可发酵纤维素含量的增加而略有下降,但仍有望获得最大的乙醇浓度(16.9 g/L)。