Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation; Siberian Federal University, Krasnoyarsk, Russian Federation.
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation.
Bioresour Technol. 2013 Oct;146:215-222. doi: 10.1016/j.biortech.2013.07.070. Epub 2013 Jul 20.
Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h(-1), on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460 h(-1), P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50 g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, γ-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties.
一株新的铜绿假单胞菌属-铜绿假单胞菌 B-10646 菌株在自养生长条件下合成聚羟基烷酸酯 (PHA)。在恒化器中，在特定流速 D=0.1h(-1)下，以 CO2 为唯一碳源，以氮、硫、磷、钾和锰作为生长限制元素，在氮缺乏时可获得最高的聚(3-羟基丁酸酯) [P(3HB)]产率。在分批自养培养中，在氧传质系数为 0.460 h(-1)的发酵罐中，P(3HB)产率达到细胞干重 (DCW)的 85%，DCW 达到 50 g/l。改变补充 PHA 前体底物 (戊酸盐、己酸盐、γ-丁内酯)的浓度和培养条件，首次在自养生长条件下生产出具有广泛不同的 3-羟基丁酸酯、4-羟基丁酸酯、3-羟基戊酸酯和 3-羟基己酸酯单体单元的 PHA 三聚物和四聚物。对高纯度 PHA 标本的研究表明，它们的物理化学和物理机械性能存在显著差异。
