Siberian Federal University , 79 Svobodnyi Av. , Krasnoyarsk 660041 , Russia.
Institute of Biophysics SB RAS , Federal Research Center "Krasnoyarsk Science Center SB RAS", 50/50 Akademgorodok , Krasnoyarsk 660036 , Russia.
Biomacromolecules. 2019 Sep 9;20(9):3261-3270. doi: 10.1021/acs.biomac.9b00295. Epub 2019 May 23.
The synthesis of polyhydroxyalkanoates (PHAs) was scaled up to pilot production in a 150-L fermenter on sugars (fructose and glucose) and purified and crude glycerol in a culture of the wild-type strain B-10646. Over 60 h of cultivation, a cell concentration of 150-160 g/L was obtained on purified glycerol and glucose; cultivation on fructose and crude glycerol resulted in a cell concentration of 130 ± 10 g/L. Polymer content and yield coefficients for the biomass were similar on all substrates (80-85 wt % and 0.29-0.33 kg biomass/kg carbon substrate, respectively). Copolymers poly(3-hydroxybutyrate-3-hydroxyvalerate) and poly(3-hydroxybutyrate--4-hydroxybutyrate) and terpolymers poly(3-hydroxybutyrate--3-hydroxyvalerate--4-hydroxybutyrate) having a decreased degree of crystallinity (36-46%) were first synthesized in the scaled-up process using B-10646 cultivated on glycerol. These results will provide the basis for scaling-up PHA synthesis in an organotrophic B-10646 culture.
聚羟基烷酸酯 (PHA) 的合成在 150-L 发酵罐中进行了扩大规模，以在野生型菌株 B-10646 的培养物中使用糖（果糖和葡萄糖）和纯化的粗甘油进行生产，并对其进行了纯化和粗甘油。在 60 多个小时的培养过程中，在纯化的甘油和葡萄糖上获得了 150-160 g/L 的细胞浓度；在果糖和粗甘油上的培养导致细胞浓度为 130±10 g/L。在所有底物上，聚合物含量和生物质的产率系数相似（分别为 80-85 wt %和 0.29-0.33 kg 生物质/kg 碳底物）。在使用甘油培养的 B-10646 进行扩大规模的过程中，首次合成了结晶度降低（36-46%）的共聚物聚（3-羟基丁酸酯-3-羟基戊酸酯）和聚（3-羟基丁酸酯-4-羟基丁酸酯）以及三元共聚物聚（3-羟基丁酸酯-3-羟基戊酸酯-4-羟基丁酸酯）。这些结果将为在有机营养型 B-10646 培养物中扩大 PHA 合成提供基础。
