Bauchet Luc, Lonjon Nicolas, Vachiery-Lahaye Florence, Boularan Alain, Privat Alain, Hugnot Jean-Philippe
Département de Neurochirurgie, Centre Hospitalier Universitaire, Hôpital Gui de Chauliac, Montpellier, France.
Methods Mol Biol. 2013;1059:87-93. doi: 10.1007/978-1-62703-574-3_8.
Our group recently provided evidence for the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. In this chapter, we review materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord from brain-dead patients who had agreed to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.
我们的团队最近提供了证据，证明成体人类脊髓中存在神经干细胞和/或祖细胞。在本章中，我们回顾了从已同意将遗体捐赠给科学以促进治疗和科学进步的脑死亡患者身上获取高质量胸腰段、腰段和骶段成体人类脊髓样本的材料和方法。还描述了从成体人类脊髓中培养前体细胞的方法。
