Bauchet Luc, Poulen Gaetan, Lonjon Nicolas, Vachiery-Lahaye Florence, Bourinet Emmanuel, Perrin Florence Evelyne, Hugnot Jean-Philippe
Department of Neurosurgery, Gui de Chauliac Hospital CHU Montpellier, Montpellier University Medical Center, Montpellier cedex 5, France.
Institute of Functional Genomics, Montpellier University, CNRS, INSERM, Montpellier, France.
Methods Mol Biol. 2022;2389:103-110. doi: 10.1007/978-1-0716-1783-0_10.
We demonstrated the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. This chapter provides materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord and human dorsal root ganglia isolated from brain-dead patients who had agreed before passing to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.
我们证明了成体人类脊髓中存在神经干细胞和/或祖细胞。本章提供了从脑死亡患者身上获取高质量胸腰段、腰段和骶段成体人类脊髓及人类背根神经节样本的材料和方法，这些脑死亡患者在生前已同意将其遗体捐赠用于科学研究以推动治疗和科学进步。文中还描述了从成体人类脊髓中培养前体细胞的方法。
