Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
PLoS One. 2013 Aug 2;8(8):e71245. doi: 10.1371/journal.pone.0071245. Print 2013.
To study the association of anergic pulmonary tuberculosis with Vδ2(+) T cells and related cytokine levels.
82 pulmonary tuberculosis patients were divided into two groups according to their purified protein derivative tuberculin skin test (TST) results: 39 with TST-negative anergic pulmonary tuberculosis and 43 with TST-positive pulmonary tuberculosis, while 40 healthy volunteers were used as control. Based on chest X-ray results, the tuberculosis lesions were scored according to their severity, with a score of ≤ 2.5 ranking as mild, 2.5-6 as moderate and ≥ 6 as severe. The Vδ2(+) T cell percentage and their expression levels of the apoptosis-related membrane surface molecule FasL in peripheral blood and bronchoalveolar lavage fluids (BALF) were analyzed by flow cytometry, while IL-2, IL-4, IL-6 and IL-10 cytokine and γ-interferon (γ-IFN) concentrations in peripheral blood were determined by ELISA.
Most of the patients with chest X-ray lesion scores higher than 6 belonged to the anergic tuberculosis group (P<0.05). Anergic pulmonary tuberculosis patients displayed reduced peripheral blood Vδ2(+) T cell counts (P<0.05) and higher FasL expression in peripheral blood Vδ2(+) T cells (P <0.05). The Vδ2(+) T cell percentages in the BALF of all tuberculosis patients were lower than in their peripheral blood (P <0.05), and IL-4 and IL-10 concentrations in peripheral blood of anergic tuberculosis patients were higher than in TST-positive tuberculosis patients and healthy controls (P <0.05).
Anergic pulmonary tuberculosis is accompanied by reduced Vδ2(+) T cell percentage, and elevated Vδ2(+) T cell FasL expression as well as enhanced IL-4 and IL-10 levels in peripheral blood.
研究无反应性肺结核与 Vδ2(+)T 细胞及相关细胞因子水平的关系。
根据纯化蛋白衍生物结核菌素皮肤试验（TST）结果将 82 例肺结核患者分为两组：TST 阴性无反应性肺结核 39 例，TST 阳性肺结核 43 例，同时选择 40 名健康志愿者作为对照组。根据胸部 X 线结果，根据病变严重程度对肺结核病变进行评分，评分≤2.5 为轻度，2.5-6 为中度，≥6 为重度。采用流式细胞术分析外周血和支气管肺泡灌洗液（BALF）中 Vδ2(+)T 细胞的百分比及其凋亡相关膜表面分子 FasL 的表达水平，采用 ELISA 法测定外周血中白细胞介素-2（IL-2）、IL-4、IL-6 和 IL-10 细胞因子及γ-干扰素（γ-IFN）浓度。
胸部 X 线病变评分高于 6 的患者大多属于无反应性结核组（P<0.05）。无反应性肺结核患者外周血 Vδ2(+)T 细胞计数减少（P<0.05），外周血 Vδ2(+)T 细胞 FasL 表达增加（P<0.05）。所有肺结核患者的 BALF 中 Vδ2(+)T 细胞百分比均低于外周血（P<0.05），无反应性肺结核患者外周血中 IL-4 和 IL-10 浓度高于 TST 阳性肺结核患者和健康对照组（P<0.05）。
无反应性肺结核患者外周血 Vδ2(+)T 细胞比例降低，Vδ2(+)T 细胞 FasL 表达增加，IL-4 和 IL-10 水平升高。
