Díaz-Rodríguez P, Pérez-Estévez A, Seoane R, González P, Serra J, Landin M
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
ISRN Pharm. 2013 Jul 7;2013:104529. doi: 10.1155/2013/104529. Print 2013.
The present work is aimed at getting a new insight into biomorphic silicon carbides (bioSiCs) as bone replacement materials. BioSiCs from a variety of precursors were produced, characterized, and loaded with a broad-spectrum antibiotic. The capacity of loaded bioSiCs for preventing and/or treating preformed S. aureus biofilms has been studied. The differences in precursor characteristics are maintained after the ceramic production process. All bioSiCs allow the loading process by capillarity, giving loaded materials with drug release profiles dependent on their microstructure. The amount of antibiotic released in liquid medium during the first six hours depends on bioSiC porosity, but it could exceed the minimum inhibitory concentration of Staphylococcus aureus, for all the materials studied, thus preventing the proliferation of bacteria. Differences in the external surface and the number and size of open external pores of bioSiCs contribute towards the variations in the effect against bacteria when experiments are carried out using solid media. The internal structure and surface properties of all the systems seem to facilitate the therapeutic activity of the antibiotic on the preformed biofilms, reducing the number of viable bacteria present in the biofilm compared to controls.
本研究旨在深入了解生物形态碳化硅（bioSiCs）作为骨替代材料的特性。制备了来自多种前驱体的bioSiCs，对其进行了表征，并负载了广谱抗生素。研究了负载bioSiCs预防和/或治疗预先形成的金黄色葡萄球菌生物膜的能力。前驱体特性的差异在陶瓷生产过程后得以保留。所有bioSiCs都可通过毛细作用进行负载过程，使负载材料的药物释放曲线取决于其微观结构。在最初六小时内，液体介质中释放的抗生素量取决于bioSiC的孔隙率，但对于所有研究材料而言，该量可能超过金黄色葡萄球菌的最低抑菌浓度，从而防止细菌增殖。当使用固体培养基进行实验时，bioSiCs外表面的差异以及开放外部孔隙的数量和大小导致了抗菌效果的变化。所有系统的内部结构和表面性质似乎都有助于抗生素对预先形成的生物膜发挥治疗作用，与对照组相比，减少了生物膜中活菌的数量。
