Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Biomed Res Int. 2013;2013:497492. doi: 10.1155/2013/497492. Epub 2013 Jul 15.
Three bioactive fractions, extracellular laccase (ex-LAC), crude endopolysaccharides (c-EPL), and a low molecular subfraction of secondary metabolites (ex-LMS), were isolated from the idiophasic cultures of the white rot fungus Cerrena unicolor. For the first time, we determined the antioxidant properties of these samples by chemiluminometric measurement (a) and assessment of the scavenging effect on ABTS (b) and the DPPH reduction rate (c). The highest reducing capability was found for the ex-LMS fraction: 39-90% for (a), 20-90% for (b), and 10-59% for (c) at the concentration of 6.25-800 µg/mL. The scavenging abilities of the C. unicolor c-EPL were between 36 and 70% for (a), 2 and 60% for (b), and 28 and 32% for (c) at the concentration of 6.25-800 µg/mL. A very high prooxidative potential was observed for the ex-LAC probes. The preliminary toxicity tests were done using the Microtox system and revealed the following percentage of the toxic effect against Vibrio fischeri: 85.37% for c-EPL, 50.67% for ex-LAC, and 99.8% for ex-LMS, respectively. The ex-LAC sample showed the antibacterial activity against Escherichia coli, c-EPL against Staphylococcus aureus, and ex-LMS against both bacterial strains, respectively, but the stronger inhibitory effect was exerted on S. aureus.
从白腐真菌 Cerrena unicolor 的间生培养物中分离出三种生物活性成分：细胞外漆酶（ex-LAC）、粗内多糖（c-EPL）和次生代谢物的低分子亚组分（ex-LMS）。我们首次通过化学发光测量（a）和对 ABTS（b）和 DPPH 还原率（c）的清除作用评估来确定这些样品的抗氧化特性。ex-LMS 级分的还原能力最高：（a）浓度为 6.25-800 µg/mL 时为 39-90%，（b）浓度为 20-90%时为 20-90%，（c）浓度为 10-59%时为 10-59%。C. unicolor c-EPL 的清除能力为 36-70%（a），2-60%（b）和 28-32%（c），浓度为 6.25-800 µg/mL。ex-LAC 探针表现出很高的促氧化潜力。使用 Microtox 系统进行了初步的毒性测试，结果表明对发光菌 Vibrio fischeri 的毒性作用百分比如下：c-EPL 为 85.37%，ex-LAC 为 50.67%，ex-LMS 为 99.8%。ex-LAC 样品对大肠杆菌具有抗菌活性，c-EPL 对金黄色葡萄球菌具有抗菌活性，ex-LMS 对两种细菌均具有抗菌活性，但对金黄色葡萄球菌的抑制作用更强。
