Department of Gastroenterology, Medical University of Lodz, Lodz, Poland.
Biomed Res Int. 2013;2013:845032. doi: 10.1155/2013/845032. Epub 2013 Jul 10.
Helicobacter pylori colonization of gastric mucosa causes pain of unknown etiology in about 15-20% of infected subjects. The aim of the present work was to determine the level of expression of enzymes involved in the synthesis of melatonin in gastric mucosa of asymptomatic and symptomatic H. pylori infected patients. To diagnose H. pylori infection, histological analysis and the urea breath test (UBT C13) were performed. The levels of mRNA expression of arylalkylamine-N-acetyltransferase (AA-NAT) and acetylserotonin methyltransferase (ASMT) were estimated in gastric mucosa with RT-PCR. The level of AA-NAT expression and AMST was decreased in H. pylori infected patients and was increased after H. pylori eradication. We conclude that decreased expression of melatonin synthesizing enzymes, AA-NAT and ASMT, in patients with symptomatic H. pylori infection returns to normal level after H. pylori eradication.
幽门螺杆菌定植于胃黏膜会导致约 15-20%的感染者出现不明原因的疼痛。本研究旨在确定无症状和有症状的幽门螺杆菌感染患者胃黏膜中参与褪黑素合成的酶的表达水平。为了诊断幽门螺杆菌感染，进行了组织学分析和尿素呼气试验（UBT C13）。通过 RT-PCR 测定胃黏膜中芳香族胺-N-乙酰转移酶（AA-NAT）和乙酰血清素甲基转移酶（ASMT）的 mRNA 表达水平。幽门螺杆菌感染患者的 AA-NAT 表达和 AMST 水平降低，幽门螺杆菌根除后增加。我们得出结论，幽门螺杆菌感染患者褪黑素合成酶 AA-NAT 和 ASMT 的表达降低，在根除幽门螺杆菌后恢复正常水平。
