Department of Chemical and Systems Biology, ‡Department of Microbiology and Immunology, and §Department of Pathology, Stanford University School of Medicine , 300 Pasteur Drive, Stanford, California 94305, United States.
Biochemistry. 2013 Sep 3;52(35):5985-96. doi: 10.1021/bi400854d. Epub 2013 Aug 21.
Elucidating the molecular and biochemical details of bacterial infections can be challenging because of the many complex interactions that exist between a pathogen and its host. Consequently, many tools have been developed to aid the study of bacterial pathogenesis. Small molecules are a valuable complement to traditional genetic techniques because they can be used to rapidly perturb genetically intractable systems and to monitor post-translationally regulated processes. Activity-based probes are a subset of small molecules that covalently label an enzyme of interest based on its catalytic mechanism. These tools allow monitoring of enzyme activation within the context of a native biological system and can be used to dissect the biochemical details of enzyme function. This review describes the development and application of activity-based probes for examining aspects of bacterial infection on both sides of the host-pathogen interface.
阐明细菌感染的分子和生化细节具有挑战性，因为病原体与其宿主之间存在许多复杂的相互作用。因此，已经开发了许多工具来辅助细菌发病机制的研究。小分子是传统遗传技术的宝贵补充，因为它们可用于快速扰乱遗传上难以处理的系统，并监测翻译后调节的过程。基于活性的探针是小分子的一个子集，根据酶的催化机制，将其共价标记为感兴趣的酶。这些工具允许在天然生物系统的背景下监测酶的激活，并可用于剖析酶功能的生化细节。这篇综述描述了用于检查宿主-病原体界面两侧细菌感染的各个方面的基于活性的探针的开发和应用。
