Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA, USA.
Nat Chem Biol. 2012 Sep;8(9):745-7. doi: 10.1038/nchembio.1023. Epub 2012 Jul 15.
Here we report AWP28, an activity-based probe that can be used to biochemically monitor caspase-1 activation in response to proinflammatory stimuli. Using AWP28, we show that apoptosis is triggered upon Salmonella enterica var. Typhimurium infection in primary mouse bone marrow macrophages lacking caspase-1. Furthermore, we report that upon Salmonella infection, inflammasome-mediated caspase-1 activity is required to bypass apoptosis in favor of proinflammatory pyroptotic cell death.
在这里，我们报告了 AWP28，这是一种基于活性的探针，可用于生化监测细胞凋亡蛋白酶-1 在应对促炎刺激时的激活。使用 AWP28，我们发现缺乏细胞凋亡蛋白酶-1 的原代小鼠骨髓巨噬细胞在感染鼠伤寒沙门氏菌后会引发细胞凋亡。此外，我们还报告称，在感染沙门氏菌后，细胞焦亡需要炎症小体介导的细胞凋亡蛋白酶-1 活性来绕过细胞凋亡，从而促进促炎细胞死亡。
