Li Yong-Kang, Dai Guang-Yi, Zhang Yu-Meng, Yao Nan
Guangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
South China National Botanical Garden, Chinese Academy of Sciences, Guangzhou 510275, China.
Plants (Basel). 2024 Dec 25;14(1):15. doi: 10.3390/plants14010015.
In plants, lipids function as structural elements and signaling molecules. Understanding lipid composition and dynamics is essential for unraveling their biological functions and metabolism. Mapping the spatiotemporal distribution of lipids in plants holds great potential for elucidating lipid biosynthetic pathways and gaining insights to guide crop genetic engineering. Recent progress in fluorescence microscopy and imaging has opened new opportunities for researchers to visualize plant lipids at high spatiotemporal resolution. In this review, we provide an up-to-date overview of the methods used to image plant lipids with fluorescence microscopy. We highlight caveats and potential limitations of these approaches and provide suggestions for optimizing their utilization. This review synthesizes current knowledge and highlights the potential of these methods to provide new insights into lipid biology.
在植物中，脂质起着结构元件和信号分子的作用。了解脂质组成和动态对于揭示其生物学功能和代谢至关重要。绘制植物中脂质的时空分布图对于阐明脂质生物合成途径和获得指导作物基因工程的见解具有巨大潜力。荧光显微镜和成像技术的最新进展为研究人员提供了新的机会，能够以高时空分辨率可视化植物脂质。在本综述中，我们提供了用于通过荧光显微镜对植物脂质进行成像的方法的最新概述。我们强调了这些方法的注意事项和潜在局限性，并为优化其应用提供了建议。本综述综合了当前的知识，并突出了这些方法为脂质生物学提供新见解的潜力。
