通过磷磁共振光谱法研究镁离子与三磷酸核苷的相互作用。
Interaction of Mg2+ ions with nucleoside triphosphates by phosphorus magnetic resonance spectroscopy.
作者信息
Son T D, Roux M, Ellenberger M
出版信息
Nucleic Acids Res. 1975 Jul;2(7):1101-10. doi: 10.1093/nar/2.7.1101.
Abstract
The interaction of Mg2+ with nucleoside triphosphates: ATP, GTP, CTP and UTP has been studied by phosphorus magnetic resonance spectroscopy in aqueous solution. The results show that these four nucleotides behave similarly. Purine and Purimidine bases have almost no effect on the phosphate groups even in the N7 pK region of ATP and GTP. The Mg2+ ion binds not to the alpha and alpha but only to the beta phosphate group. The fixation is stronger at neutral pH than at acid pH.
摘要
通过磷磁共振光谱法在水溶液中研究了Mg2+与三磷酸核苷（ATP、GTP、CTP和UTP）的相互作用。结果表明，这四种核苷酸表现相似。嘌呤和嘧啶碱基即使在ATP和GTP的N7 pK区域对磷酸基团也几乎没有影响。Mg2+离子不与α和α结合，只与β磷酸基团结合。在中性pH下的结合比在酸性pH下更强。
相似文献
1
Interaction of Mg2+ ions with nucleoside triphosphates by phosphorus magnetic resonance spectroscopy.通过磷磁共振光谱法研究镁离子与三磷酸核苷的相互作用。
Nucleic Acids Res. 1975 Jul;2(7):1101-10. doi: 10.1093/nar/2.7.1101.
2
A 31P-NMR study of the interaction of Mg2+ ions with nucleoside diphosphates.一项关于镁离子与核苷二磷酸相互作用的31P核磁共振研究。
Nucleic Acids Res. 1977 Feb;4(2):397-403. doi: 10.1093/nar/4.2.397.
3
A phosphorus-magnetic-resonance study of the interaction of Mg2+ with adenyl-5'-yl imidodiphosphate. Binding sites of Mg2+ ion on the phosphate chain.Mg2+与腺苷-5'-基亚氨基二磷酸相互作用的磷磁共振研究。Mg2+离子在磷酸链上的结合位点。
Eur J Biochem. 1977 Jun 1;76(1):245-9. doi: 10.1111/j.1432-1033.1977.tb11589.x.
4
Inhibition of phosphodiesterases from Bacillus subtilis by nucleoside triphosphates.
Biochemistry. 1974 Nov 19;13(24):5008-11. doi: 10.1021/bi00721a022.
5
Initiation characteristics for the synthesis of five T4 phage-specific messenger RNAs in vitro.体外合成五种T4噬菌体特异性信使核糖核酸的起始特征
Proc Natl Acad Sci U S A. 1974 Feb;71(2):422-6. doi: 10.1073/pnas.71.2.422.
6
Nucleoside triphosphates inhibit ADP, collagen, and epinephrine-induced platelet aggregation: role of P2Y₁ and P2Y₁₂ receptors.核苷三磷酸抑制 ADP、胶原和肾上腺素诱导的血小板聚集：P2Y₁ 和 P2Y₁₂ 受体的作用。
Thromb Res. 2013 Nov;132(5):548-57. doi: 10.1016/j.thromres.2013.08.021. Epub 2013 Sep 1.
7
Comparison of the metal-ion-promoted dephosphorylation of the 5'-triphosphates of adenosine, inosine, guanosine and cytidine by Mn2+, Ni2+ and Zn2+ in binary and ternary complexes.二元和三元络合物中Mn2+、Ni2+和Zn2+对腺苷、肌苷、鸟苷和胞苷5'-三磷酸的金属离子促进去磷酸化作用的比较
Eur J Biochem. 1976 Apr 1;63(2):569-81. doi: 10.1111/j.1432-1033.1976.tb10261.x.
8
Stabilities and isomeric equilibria in solutions of monomeric metal-ion complexes of guanosine 5'-triphosphate (GTP4-) and inosine 5'-triphosphate (ITP4-) in comparison with those of adenosine 5'-triphosphate (ATP4-).鸟苷 5'-三磷酸（GTP4-）和肌苷 5'-三磷酸（ITP4-）的单体金属离子络合物溶液中的稳定性和异构平衡，与腺苷 5'-三磷酸（ATP4-）的稳定性和异构平衡的比较。
Chemistry. 2001 Sep 3;7(17):3729-37. doi: 10.1002/1521-3765(20010903)7:17<3729::aid-chem3729>3.0.co;2-e.
9
The interaction of histone IV with nucleoside triphosphates.组蛋白IV与三磷酸核苷的相互作用。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2687-90. doi: 10.1073/pnas.69.9.2687.
10
Continuous electron spin resonance detection of biochemical reactions of nucleoside triphosphates.
Anal Biochem. 1977 Feb;77(2):413-8. doi: 10.1016/0003-2697(77)90254-8.
引用本文的文献
1
In situ NMR measurement of macromolecule-bound metal ion concentrations.大分子结合金属离子浓度的原位核磁共振测量。
J Biomol NMR. 2016 Apr;64(4):269-73. doi: 10.1007/s10858-016-0031-3. Epub 2016 Apr 23.
2
Parametrization of DFTB3/3OB for magnesium and zinc for chemical and biological applications.用于化学和生物应用的镁和锌的DFTB3/3OB参数化
J Phys Chem B. 2015 Jan 22;119(3):1062-82. doi: 10.1021/jp506557r. Epub 2014 Sep 16.
3
Stabilities of the Divalent Metal Ion Complexes of a Short-Chain Polyphosphate Anion and Its Imino Derivative.短链多磷酸根阴离子及其亚氨基衍生物的二价金属离子配合物的稳定性
J Solution Chem. 2013;42(11):2104-2118. doi: 10.1007/s10953-013-0099-2. Epub 2013 Nov 7.
4
Identification and characterization of a novel high affinity metal-binding site in the hammerhead ribozyme.锤头状核酶中一个新型高亲和力金属结合位点的鉴定与表征
RNA. 1999 Aug;5(8):1099-104. doi: 10.1017/s1355838299990738.
5
31P NMR studies of human soleus and gastrocnemius show differences in the J gamma beta coupling constant of ATP and in intracellular free magnesium.对人类比目鱼肌和腓肠肌的31P核磁共振研究表明，ATP的Jγβ耦合常数和细胞内游离镁存在差异。
MAGMA. 1996 Mar;4(1):47-53. doi: 10.1007/BF01759779.
6
Tendencies of 31P chemical shifts changes in NMR spectra of nucleotide derivatives.核苷酸衍生物核磁共振谱中³¹P化学位移变化的趋势。
Nucleic Acids Res. 1984 Jul 25;12(14):5547-66. doi: 10.1093/nar/12.14.5547.
7
Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.蟾蜍视网膜的磷-31核磁共振波谱分析
Biophys J. 1989 Sep;56(3):447-52. doi: 10.1016/S0006-3495(89)82691-8.
8
Adenine nucleotide storage and secretion in platelets as studied by 31P nuclear magnetic resonance.通过31P核磁共振研究血小板中腺嘌呤核苷酸的储存与分泌
Proc Natl Acad Sci U S A. 1979 May;76(5):2227-31. doi: 10.1073/pnas.76.5.2227.
9
A 31P-NMR study of the interaction of Mg2+ ions with nucleoside diphosphates.一项关于镁离子与核苷二磷酸相互作用的31P核磁共振研究。
Nucleic Acids Res. 1977 Feb;4(2):397-403. doi: 10.1093/nar/4.2.397.
本文引用的文献
1
MAGNETIC RESONANCE STUDIES OF METAL ACTIVATION OF ENZYMIC REACTIONS OF NUCLEOTIDES AND OTHER PHOSPHATE SUBSTRATES.核苷酸及其他磷酸盐底物酶促反应的金属激活的磁共振研究。
Biochemistry. 1963 Jul-Aug;2:623-9. doi: 10.1021/bi00904a001.
2
3
Bond cleavage in enzymic hydrolysis of nucleoside triphosphate to nucleoside monophosphate and inorganic pyrophosphate.核苷三磷酸酶解为核苷一磷酸和无机焦磷酸过程中的键断裂。
Biochim Biophys Acta. 1960 Jan 15;37:344-6. doi: 10.1016/0006-3002(60)90244-4.
4
Mechanism of enzyme action.酶的作用机制。
Annu Rev Biochem. 1960;29:15-44. doi: 10.1146/annurev.bi.29.070160.000311.
5
Nucleotide specificity and conformation of the active site of creatine kinase. Magnetic resonance and sulfhydryl reactivity studies.肌酸激酶活性位点的核苷酸特异性和构象。磁共振与巯基反应性研究。
J Biol Chem. 1966 Jul 10;241(13):3116-25.
6
Nuclear magnetic resonance study of metal-ion binding to adenosine triphosphate. II. Proton studies.
J Chem Phys. 1965 Nov 1;43(9):3133-43. doi: 10.1063/1.1697287.
7
Nuclear magnetic resonance study of metal-ion binding to adenosine triphosphate. I. 31-P studies.
J Chem Phys. 1965 Nov 1;43(9):3123-32. doi: 10.1063/1.1697286.
8
Metal ion binding to adenosine triphosphate. 3. A kinetic analysis.金属离子与三磷酸腺苷的结合。3. 动力学分析。
J Am Chem Soc. 1968 Dec 4;90(25):7110-8. doi: 10.1021/ja01027a041.
9
A proton magnetic resonance study of metal ion-adenine ring interactions in metal ion complexes with adenosine triphosphate.金属离子与三磷酸腺苷形成的金属离子配合物中金属离子 - 腺嘌呤环相互作用的质子磁共振研究。
Biochemistry. 1971 Mar 2;10(5):843-51. doi: 10.1021/bi00781a018.
10
13C relaxation studies on the manganese(II)-adenosine 5'-triphosphate complex in solution.
J Am Chem Soc. 1974 Mar 20;96(6):1834-9. doi: 10.1021/ja00813a030.
Zotero 插件