通过磷磁共振光谱法研究镁离子与三磷酸核苷的相互作用。
Interaction of Mg2+ ions with nucleoside triphosphates by phosphorus magnetic resonance spectroscopy.
作者信息
Son T D, Roux M, Ellenberger M
出版信息
Nucleic Acids Res. 1975 Jul;2(7):1101-10. doi: 10.1093/nar/2.7.1101.
Abstract
The interaction of Mg2+ with nucleoside triphosphates: ATP, GTP, CTP and UTP has been studied by phosphorus magnetic resonance spectroscopy in aqueous solution. The results show that these four nucleotides behave similarly. Purine and Purimidine bases have almost no effect on the phosphate groups even in the N7 pK region of ATP and GTP. The Mg2+ ion binds not to the alpha and alpha but only to the beta phosphate group. The fixation is stronger at neutral pH than at acid pH.
摘要
通过磷磁共振光谱法在水溶液中研究了Mg2+与三磷酸核苷（ATP、GTP、CTP和UTP）的相互作用。结果表明，这四种核苷酸表现相似。嘌呤和嘧啶碱基即使在ATP和GTP的N7 pK区域对磷酸基团也几乎没有影响。Mg2+离子不与α和α结合，只与β磷酸基团结合。在中性pH下的结合比在酸性pH下更强。
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