Avramidou Eleni, Sarri Efi, Ganopoulos Ioannis, Madesis Panagiotis, Kougiteas Leonidas, Papadopoulou Evgenia-Anna, Aliferis Konstantinos A, Abraham Eleni M, Tani Eleni
Department of Forestry and Natural Environment, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Institute of Applied Bioscience, CERTH, Thermi, 57001 Thessaloniki, Greece.
Plants (Basel). 2023 Feb 17;12(4):908. doi: 10.3390/plants12040908.
L. (faba bean) is one of the most promising pulse crops due to its nutritional value and high nitrogen fixation capacity. The aim of the present study was to compare the genetic diversity and the seed metabolite profiles of five genetic materials of faba bean. Specifically, three newly developed advanced lines (KK18, KK14 and KK10) and two commercial cultivars (POLIKARPI and TANAGRA), were evaluated for this purpose. Genetic diversity among populations was assessed by SCoT molecular markers. Through UPGMA dendrogram, genetic distances between populations were estimated. Untargeted metabolomics analysis of the seeds was performed employing GC/EI/MS. The cultivar POLYKARPI exhibited the highest polymorphism. All varieties showed a higher within-cultivars and advanced lines variability than between. POLYKARPI and KK14 had the lowest genetic distances, while KK18 and TANAGRA presented the highest ones. The advanced line KK18 displayed the best nutritional profile, the highest concentration of desirable metabolites (lactic acid and trehalose), the lowest concentration of anti-nutritional factors (oxalic acid) and the lowest concentration of saturated fatty acids (palmitic and stearic acid). According to the results of the present study, KK18 line is a very promising material for further exploration and utilization in breeding programs.
蚕豆因其营养价值和高固氮能力，是最具潜力的豆类作物之一。本研究的目的是比较五种蚕豆遗传材料的遗传多样性和种子代谢物谱。具体而言，为此评估了三个新培育的高代品系（KK18、KK14和KK10）和两个商业品种（POLIKARPI和TANAGRA）。通过SCoT分子标记评估群体间的遗传多样性。通过UPGMA聚类图估计群体间的遗传距离。采用GC/EI/MS对种子进行非靶向代谢组学分析。POLYKARPI品种表现出最高的多态性。所有品种在品种内和高代品系内的变异性均高于品种间。POLYKARPI和KK14的遗传距离最低，而KK18和TANAGRA的遗传距离最高。高代品系KK18表现出最佳的营养状况，理想代谢物（乳酸和海藻糖）浓度最高，抗营养因子（草酸）浓度最低，饱和脂肪酸（棕榈酸和硬脂酸）浓度最低。根据本研究结果，KK18品系是育种计划中进一步探索和利用的非常有前景的材料。
