Feng Shangguo, He Refeng, Yang Sai, Chen Zhe, Jiang Mengying, Lu Jiangjie, Wang Huizhong
College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha 410128, China; Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310018, China.
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310018, China.
Gene. 2015 Aug 10;567(2):182-8. doi: 10.1016/j.gene.2015.04.076. Epub 2015 Apr 30.
Two molecular marker systems, start codon targeted (SCoT) and target region amplification polymorphism (TRAP), were used for genetic relationship analysis of 36 Dendrobium species collected from China. Twenty-two selected SCoT primers produced 337 loci, of which 324 (96%) were polymorphic, whereas 13 TRAP primer combinations produced a total of 510 loci, with 500 (97.8%) of them being polymorphic. An average polymorphism information content of 0.953 and 0.983 was detected using the SCoT and TRAP primers, respectively, showing that a high degree of genetic diversity exists among Chinese Dendrobium species. The partition of clusters in the unweighted pair group method with arithmetic mean dendrogram and principal coordinate analysis plot based on the SCoT and TRAP markers was similar and clustered the 36 Dendrobium species into four main groups. Our results will provide useful information for resource protection and will also be useful to improve the current Dendrobium breeding programs. Our results also demonstrate that SCoT and TRAP markers are informative and can be used to evaluate genetic relationships between Dendrobium species.
使用起始密码子靶向（SCoT）和目标区域扩增多态性（TRAP）这两种分子标记系统，对从中国收集的36种石斛属植物进行亲缘关系分析。22条选定的SCoT引物产生了337个位点，其中324个（96%）具有多态性；而13个TRAP引物组合共产生了510个位点，其中500个（97.8%）具有多态性。分别使用SCoT和TRAP引物检测到的平均多态性信息含量为0.953和0.983，表明中国石斛属植物之间存在高度的遗传多样性。基于SCoT和TRAP标记的算术平均非加权组对法树状图和主坐标分析图中的聚类划分相似，将36种石斛属植物聚类为四个主要类群。我们的结果将为资源保护提供有用信息，也有助于改进当前的石斛属植物育种计划。我们的结果还表明，SCoT和TRAP标记具有信息性，可用于评估石斛属植物之间的亲缘关系。
