State Key Laboratory of Virology and China Center for Virus Culture Collection, Chinese Academy of Sciences, Wuhan 430071, PR China.
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumuqi, Xinjiang 830046, PR China.
J Gen Virol. 2013 Nov;94(Pt 11):2536-2548. doi: 10.1099/vir.0.056374-0. Epub 2013 Aug 12.
Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus with high pathogenicity to humans. CCHFV contains a three-segment [small (S), medium (M) and large (L)] genome and is prone to reassortment. Investigation of identified reassortment events can yield insight into the evolutionary history of the virus, while migration events reflect its geographical dissemination. While many studies have already considered these issues, they have investigated small numbers of isolates and lack statistical support for their findings. Here, we consider a larger set of 30 full genomes to investigate reassortment using recombination methods, as well as two sets of partial S segments comprising 393 isolates, reflecting a broader geographical range, to investigate migration events. Phylogenetic analysis revealed that the S segment showed strong geographical subdivision, but this was less apparent in the M and L segments. A total of 16 reassortment events with 22 isolates were identified with strong statistical support. Migration analysis on the partial S segments identified both long- and short-range migration events that spanned the entire geographical region in which the CCHFV has been isolated, reflecting the complex processes associated with the dissemination of the virus.
克里米亚-刚果出血热病毒（CCHFV）是一种具有高致病性的蜱传病毒。CCHFV 包含一个三片段[小（S）、中（M）和大（L）]基因组，容易发生重组。对已确定的重组事件的调查可以深入了解病毒的进化历史，而迁移事件则反映了其地理传播。虽然已经有许多研究考虑了这些问题，但它们只调查了少数分离株，而且其发现缺乏统计学支持。在这里，我们考虑了更大的一组 30 个完整基因组，使用重组方法研究重组，以及两组包含 393 个分离株的部分 S 片段，反映了更广泛的地理范围，以研究迁移事件。系统发育分析表明，S 片段显示出强烈的地理细分，但在 M 和 L 片段中则不太明显。共鉴定出 16 个具有强烈统计学支持的重组事件和 22 个分离株。对部分 S 片段的迁移分析确定了长距离和短距离迁移事件，这些事件跨越了 CCHFV 已分离的整个地理区域，反映了与病毒传播相关的复杂过程。
