Comparative Oncology Laboratory, School of Veterinary Medicine, University of California Davis, Davis, California, United States of America.
PLoS One. 2013 Aug 7;8(8):e71724. doi: 10.1371/journal.pone.0071724. Print 2013.
p63, a transcription factor and p53 family protein, plays a crucial role in tumor suppression and development of various epithelial tissues. While p63 expression is controlled mostly by post-translational modifications, recent studies indicate that transcriptional and posttranscriptional regulations are essential for proper p63 expression. Here, we investigated the regulation of p63 expression by poly (C)-binding protein 1 (PCBP1, also known as hnRNP-E1 and αCP1). We found that knockdown of PCBP1 decreases the level of p63 transcript and protein. We also found that PCBP1 regulates the stability of p63 mRNA via binding to p63 3'UTR. Additionally, we found that a CU-rich element (CUE) in p63 3'UTR is bound by and responsive to PCBP1. Together, we conclude that PCBP1 regulates p63 expression via mRNA stability.
p63 是一种转录因子和 p53 家族蛋白，在肿瘤抑制和各种上皮组织的发育中起着关键作用。虽然 p63 的表达主要受翻译后修饰的控制，但最近的研究表明转录和转录后调控对 p63 的正确表达至关重要。在这里，我们研究了多聚（C）结合蛋白 1（PCBP1，也称为 hnRNP-E1 和 αCP1）对 p63 表达的调节。我们发现，PCBP1 的敲低降低了 p63 转录本和蛋白的水平。我们还发现，PCBP1 通过结合 p63 3'UTR 来调节 p63 mRNA 的稳定性。此外，我们发现 p63 3'UTR 中的 CU 丰富元件（CUE）结合并响应 PCBP1。综上所述，我们得出结论，PCBP1 通过 mRNA 稳定性来调节 p63 的表达。
