Laboratory of Food Chemistry, Wageningen University , P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
J Agric Food Chem. 2013 Sep 11;61(36):8657-67. doi: 10.1021/jf4020203. Epub 2013 Aug 28.
The isoflavonoid profile of soybean was altered in different ways by stimulation of defense response upon germination. The combination of simultaneous germination and induction by Rhizopus oryzae increased the total isoflavonoid content of soybeans over 2-fold. Pterocarpans became the predominant isoflavonoids, up to 50% (w/w) of total isoflavonoids. To modulate both isoflavonoid content and composition further, the treatment was extended with wounding or light stimuli. The total isoflavonoid content could be increased over 3-fold compared to untreated beans by growing fungus-elicited soybean seedlings in light, whereas wounding was less effective. Interestingly, light altered the composition of prenylated pterocarpans by mediating the position of prenylation. The 2-prenylated pterocarpan level increased 2-fold, whereas that of 4-prenylated pterocarpan remained similar. Taken together, fungus was the most effective elicitor to alter the isoflavonoid content and composition of soybean seedlings, the impact of which can be further enhanced and mediated by additional stimuli, particularly light.
大豆中的异黄酮谱在萌发时防御反应的刺激下以不同的方式发生改变。同时萌发和米根霉诱导的组合使大豆中的总异黄酮含量增加了 2 倍以上。紫檀烷成为主要的异黄酮，占总异黄酮的 50%（w/w）。为了进一步调节异黄酮的含量和组成，用创伤或光照刺激来延长处理时间。与未处理的大豆相比，在光照下生长真菌诱导的大豆幼苗可使总异黄酮含量增加 3 倍以上，而创伤的效果则较差。有趣的是，光通过调节异戊烯基化的位置来改变类异戊二烯紫檀烷的组成。2-异戊烯基化紫檀烷的水平增加了 2 倍，而 4-异戊烯基化紫檀烷的水平保持不变。总之，真菌是最有效的诱导因子，可以改变大豆幼苗的异黄酮含量和组成，而其他刺激物，特别是光照，可以进一步增强和介导这种影响。
