Jahan Md Asraful, Kovinich Nik
a Division of Plant and Soil Sciences , West Virginia University , Morgantown , WV , USA.
Plant Signal Behav. 2019;14(7):1604018. doi: 10.1080/15592324.2019.1604018. Epub 2019 Apr 15.
Glyceollins are the major pathogen- and stress-inducible natural products (phytoalexins) of soybean that possess broad-spectrum anticancer and neuroprotective properties. Yet like other phytoalexins, glyceollins are difficult to obtain because they are typically biosynthesized only transiently and in low amounts in plant tissues. We recently identified acidity stress (pH 3.0 growth medium) as an elicitor that exerted prolonged (week-long) inductive effects on glyceollin biosynthesis and identified the NAC family TF gene that activates glyceollin biosynthesis in response to acidity stress or WGE from the soybean pathogen was annotated as an SAR gene and SAR genes were statistically overrepresented in the transcriptomic response to acidity stress suggesting that acidity stress triggers the systemic elicitation of glyceollin biosynthesis. Here, we demonstrate that acidity stress acts as a systemic elicitor when provided to soybean roots. Acidity stress preferentially elicited specific glyceollins in different soybean organs with exceptionally high yields of glyceollin I in root tissues.
大豆抗毒素是大豆中主要的病原体和胁迫诱导型天然产物（植保素），具有广谱抗癌和神经保护特性。然而，与其他植保素一样，大豆抗毒素很难获得，因为它们通常仅在植物组织中短暂生物合成且含量很低。我们最近确定酸性胁迫（pH 3.0生长培养基）是一种诱导物，对大豆抗毒素生物合成具有长期（长达一周）的诱导作用，并鉴定出响应酸性胁迫或大豆病原体WGE激活大豆抗毒素生物合成的NAC家族转录因子基因，该基因被注释为一个系统获得性抗性（SAR）基因，并且在对酸性胁迫的转录组反应中，SAR基因在统计学上显著富集，这表明酸性胁迫触发了大豆抗毒素生物合成的系统性诱导。在此，我们证明当将酸性胁迫施加于大豆根系时，其可作为一种系统性诱导物。酸性胁迫优先在不同大豆器官中诱导产生特定的大豆抗毒素，其中根组织中大豆抗毒素I的产量极高。
