Liu Meiyan, Du Bing, Li Chunhong, Zhao Yanbin, Meng Qingwei, Cai Li
Department of Medicine, Affiliated Tumor Hospital of Harbin Medical University, 150081 Harbin, China.
Zhongguo Fei Ai Za Zhi. 2013 Aug 20;16(8):417-21. doi: 10.3779/j.issn.1009-3419.2013.08.05.
Galectin-3 is involved in many physical and pathologic process, and is closely correlated with tumorigenesis, development and metastasis. The aim of this study is to investigate the gene expression and significance of Galectin-3 in normal lung tissue and non-small cell lung cancer (NSCLC) tissue.
Gene expression was examined using the immunohistochemical S-P method in 62 NSCLC cases and in 17 cases with normal lung tissue. RT-PCR verified the expression of Galectin-3 at the transcriptional level.
The expression rate of Galectin-3 in NSCLC tissue was 83.8% (52/62), which was significantly higher than that in normal lung tissue (P<0.01). Galectin-3 expression was not correlated with sex (χ²=0.113, P>0.05), age (χ²=0.220, P>0.05), and histological type (χ²=0.012, P>0.05), but was negatively correlated with pathological differentiation (r=-0.292, P<0.05) and positively correlated with the clinical stage (r=0.336, P<0.05) in NSCLC tissue. RT-PCR results showed that the expression level of Galectin-3 in NSCLC tissue was significantly higher than in normal lung tissue.
Galectin-3 may be useful in lung cancer diagnosis and may increase diagnosis rate when combined with other lung cancer markers.
半乳糖凝集素-3参与多种生理和病理过程，与肿瘤的发生、发展及转移密切相关。本研究旨在探讨半乳糖凝集素-3在正常肺组织和非小细胞肺癌（NSCLC）组织中的基因表达及意义。
采用免疫组织化学S-P法检测62例NSCLC患者及17例正常肺组织中半乳糖凝集素-3的基因表达。RT-PCR从转录水平验证半乳糖凝集素-3的表达。
NSCLC组织中半乳糖凝集素-3的表达率为83.8%（52/62），显著高于正常肺组织（P<0.01）。NSCLC组织中半乳糖凝集素-3的表达与性别（χ²=0.113，P>0.05）、年龄（χ²=0.220，P>0.05）及组织学类型（χ²=0.012，P>0.05）无关，但与病理分化呈负相关（r=-0.292，P<0.05），与临床分期呈正相关（r=0.336，P<0.05）。RT-PCR结果显示，NSCLC组织中半乳糖凝集素-3的表达水平显著高于正常肺组织。
半乳糖凝集素-3可能有助于肺癌诊断，与其他肺癌标志物联合使用时可能提高诊断率。
