Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan.
Lipids Health Dis. 2013 Aug 15;12:124. doi: 10.1186/1476-511X-12-124.
Various flavonoids obtained from the genus Cirsium have been reported to exhibit beneficial effects on health. The present study evaluated the antiobesity effects of Cirsium brevicaule A. GRAY leaf (CL) by using 3T3-L1 cells and C57BL/6 mice that were fed a high-fat diet (HFD).
Dried CL powder was serially extracted with solvents of various polarities, and these extracts were tested for antiadipogenic activity using 3T3-L1 adipocytes. Mice were fed experimental HFD supplemented with dried CL powder for 4 wk. Lipid levels and mRNA levels of genes related to lipid metabolism were determined in 3T3-L1 adipocytes and the white adipose tissue (WAT) and liver of mice fed on a HFD.
Treatment of 3T3-L1 adipocytes with a hexane extract of CL significantly reduced cellular lipid accumulation and expression of the fatty acid synthase (FASN) gene. Dietary CL reduced the serum levels of non-esterified fatty acids in HFD-fed mice. Significant decreases in subcutaneous WAT weight and associated FASN gene expression were observed in the mice fed the experimental CL diet. Dietary CL also reduced the hepatic lipid and serum levels of a hepatopathic indicator in the HFD-fed mice. A significant reduction in mRNA levels of FASN and HMG-CoA reductase were observed in the livers of the CL-diet group. Dietary CL, on the other hand, increased in the hepatic mRNA levels of genes related to β-oxidation, namely peroxisome proliferator-activated receptor α, calnitine palmitoyltrasferase 1A, and uncoupling protein 2. Expression of the insulin receptor gene was also significantly increased in the livers of mice-fed the CL diet.
The present study therefore demonstrated that CL suppresses lipid accumulation in the WAT and liver partly through inhibiting mRNA levels of FASN gene and enhancing the lipolysis-related gene expression.
已报道来自蓟属的各种类黄酮具有有益的健康效应。本研究通过使用 3T3-L1 细胞和高脂肪饮食（HFD）喂养的 C57BL/6 小鼠，评估了短葶飞蓬（CL）叶的抗肥胖作用。
用不同极性溶剂连续提取干燥的 CL 粉末，并使用 3T3-L1 脂肪细胞测试其抗脂肪生成活性。将小鼠用补充有干燥的 CL 粉末的实验性 HFD 喂养 4 周。测定 3T3-L1 脂肪细胞和 HFD 喂养的小鼠白色脂肪组织（WAT）和肝脏中与脂质代谢相关的基因的脂质水平和 mRNA 水平。
CL 的正己烷提取物处理 3T3-L1 脂肪细胞显著减少了细胞内脂质积累和脂肪酸合酶（FASN）基因的表达。CL 饮食降低了 HFD 喂养小鼠的血清非酯化脂肪酸水平。在给予实验性 CL 饮食的小鼠中，皮下 WAT 重量及其相关 FASN 基因表达显著降低。CL 饮食还降低了 HFD 喂养小鼠的肝脂质和血清肝病变指标水平。CL 饮食组肝中 FASN 和 HMG-CoA 还原酶的 mRNA 水平显著降低。另一方面，CL 饮食增加了与β氧化相关的基因，即过氧化物酶体增殖物激活受体α、肉毒碱棕榈酰转移酶 1A 和解偶联蛋白 2 的肝 mRNA 水平。给予 CL 饮食的小鼠肝脏中胰岛素受体基因的表达也显著增加。
因此，本研究表明 CL 通过抑制 FASN 基因的 mRNA 水平和增强与脂肪分解相关的基因表达，部分抑制 WAT 和肝脏中的脂质积累。
