College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Molecules. 2013 Aug 13;18(8):9717-26. doi: 10.3390/molecules18089717.
Resveratrol (RV), a dietary antioxidant polyphenolic compound found in grapes and red wine, exerts a wide variety of pharmacological activities. However, lower content in plants compared with polydatin (PD, the glucoside of RV) limits its application in the food and pharmaceutical industries. In this paper, we carried out efficient biotransformation of PD to RV with 100% conversion yield by snailase hydrolysis. Moreover, response surface methodology (RSM) was used to optimize the effects of the reaction temperature, enzyme load, and reaction time on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted RV yield values. The optimum preparation conditions were as follows: temperature of 62.0 °C, enzyme load of 6.6%, and reaction time of 96 min. The proposed method may be highly applicable for the enzymatic preparation of RV for medicinal purposes.
白藜芦醇（RV）是一种存在于葡萄和红酒中的膳食抗氧化多酚化合物，具有广泛的药理活性。然而，与白藜芦醇苷（PD，RV 的糖苷）相比，其在食品和制药工业中的含量较低，限制了其应用。在本文中，我们利用蜗牛酶水解将 PD 高效转化为 RV，转化率达到 100%。此外，还采用响应面法（RSM）优化了反应温度、酶用量和反应时间对转化过程的影响。RSM 模型的验证结果表明，实验值和预测 RV 产率值吻合较好。最佳制备条件为：温度 62.0°C，酶用量 6.6%，反应时间 96 min。该方法可能高度适用于药用 RV 的酶法制备。
