Jeandet Philippe, Delaunois Bertrand, Aziz Aziz, Donnez David, Vasserot Yann, Cordelier Sylvain, Courot Eric
Laboratory of Enology and Applied Chemistry, Research Unit "Vines and Wines of Champagne," UPRES EA 4707, Faculty of Sciences, University of Reims, P.O. Box 1039, 51687 Reims Cedex 02, France.
J Biomed Biotechnol. 2012;2012:579089. doi: 10.1155/2012/579089. Epub 2012 May 13.
Resveratrol, a stilbenic compound deriving from the phenyalanine/polymalonate route, being stilbene synthase the last and key enzyme of this pathway, recently has become the focus of a number of studies in medicine and plant physiology. Increased demand for this molecule for nutraceutical, cosmetic and possibly pharmaceutic uses, makes its production a necessity. In this context, the use of biotechnology through recombinant microorganisms and plants is particularly promising. Interesting results can indeed arise from the potential of genetically modified microorganisms as an alternative mechanism for producing resveratrol. Strategies used to tailoring yeast as they do not possess the genes that encode for the resveratrol pathway, will be described. On the other hand, most interest has centered in recent years, on STS gene transfer experiments from various origins to the genome of numerous plants. This work also presents a comprehensive review on plant molecular engineering with the STS gene, resulting in disease resistance against microorganisms and the enhancement of the antioxidant activities of several fruits in transgenic lines.
白藜芦醇是一种源自苯丙氨酸/丙二酸途径的芪类化合物，而芪合酶是该途径的最后一个关键酶，最近它已成为医学和植物生理学众多研究的焦点。营养保健品、化妆品以及可能的药物用途对这种分子的需求不断增加，使得其生产成为必要。在这种背景下，通过重组微生物和植物利用生物技术尤其具有前景。转基因微生物作为生产白藜芦醇的替代机制的潜力确实能够产生有趣的结果。本文将描述为改造不具备编码白藜芦醇途径基因的酵母所采用的策略。另一方面，近年来，大多数兴趣集中在将来自各种来源的芪合酶基因（STS基因）转移到众多植物基因组的实验上。这项工作还全面综述了利用STS基因进行植物分子工程，从而使转基因品系获得抗微生物病害能力以及增强几种果实的抗氧化活性。
