State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, China.
Guangxi Key Laboratory of Bio-Refinery, National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, China.
Molecules. 2022 Feb 23;27(5):1514. doi: 10.3390/molecules27051514.
Resveratrol, an ingredient of traditional Chinese medicine, has beneficial effects on human health and huge potential for application in modern medicine. Polydatin is extracted from plants and then deglycosylated into resveratrol; enzymatic methods are preferred for this reaction. In this study, a β-D-glucosidase from showed high efficiency in transforming polydatin into resveratrol and was tolerant toward organic solvents. Applying this enzyme in a biphasic transformation system resulted in 95.3% conversion of 20% concentration crude polydatin to resveratrol in 4 h. We thus report a new method for high-efficiency, clean production of resveratrol.
白藜芦醇是一种中药成分，对人类健康有有益影响，在现代医学中有巨大的应用潜力。虎杖苷是从植物中提取出来的，然后通过去糖基化反应转化为白藜芦醇；该反应更倾向于使用酶法进行。在这项研究中，来自 的 β-D-葡萄糖苷酶在将虎杖苷转化为白藜芦醇方面表现出很高的效率，并且对有机溶剂具有耐受性。在两相转化系统中应用这种酶可以在 4 小时内将 20%浓度的粗虎杖苷转化为 95.3%的白藜芦醇。因此，我们报告了一种高效、清洁生产白藜芦醇的新方法。
