a Sichuan Provincial Key-Laboratory of Protection and Utilization of Animal Genetic Resources in Qinghai-Tibet Plateau, Southwest University for Nationalities , Chengdu , P. R. China.
Anim Biotechnol. 2013;24(4):307-11. doi: 10.1080/10495398.2013.790824.
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have a central role in follicle growth and maturation, but no clear differences between breeds with different ovulation rates have been found. Therefore, this study investigated mRNA expression of FSHβ, LHβ, FSH receptor (FSHR), LH receptor (LHR), and estrogen receptor-β (ERβ) genes in prolific Lezhi black (LB) goats and nonprolific Tibetan (TB) goats by real-time PCR. Follicles and pituitaries were recovered from goats at 12-24 h after onset of estrus. Real-time PCR analysis revealed that the expression levels of FSHβ and LHβ mRNA were significantly higher (p < 0.01) in pituitary of LB than in TB does, but the expression levels of FSHR and LHR mRNA in follicle of TB were greater (p < 0.05). Expression level of follicular ER β was not different between the two breeds. Data provide evidence that the greater ovulation rate in the LB goat as compared to the TB breed is associated with a greater gonadotropin expression during follicular phase.
卵泡刺激素（FSH）和黄体生成素（LH）在卵泡生长和成熟中起着核心作用，但尚未发现排卵率不同的品种之间存在明显差异。因此，本研究通过实时 PCR 检测了高产乐至黑（LB）山羊和非高产西藏（TB）山羊的 FSHβ、LHβ、FSH 受体（FSHR）、LH 受体（LHR）和雌激素受体-β（ERβ）基因的 mRNA 表达。发情后 12-24 小时从山羊中回收卵泡和垂体。实时 PCR 分析显示，LB 山羊垂体中 FSHβ 和 LHβ mRNA 的表达水平显著高于 TB 山羊（p < 0.01），但 TB 山羊卵泡中 FSHR 和 LHR mRNA 的表达水平更高（p < 0.05）。两种品种的卵泡 ERβ 表达水平无差异。数据表明，LB 山羊的排卵率高于 TB 品种，这与卵泡期促性腺激素表达增加有关。
