DNA Database Laboratory, Division of Identification and Forensic Science, Israel Police, National H.Q., Jerusalem, Israel.
Forensic Sci Int Genet. 2013 Sep;7(5):494-8. doi: 10.1016/j.fsigen.2013.04.011. Epub 2013 Jun 28.
This report demonstrates the limits of DNA identification when siblings are involved. The Israeli DNA database routinely amplifies suspects samples using the PowerPlex(®) ESI16 system (Promega). While uploading a series of suspects into the database software, we found an unusual high number of shared alleles between two suspects 31 out of 32 alleles. Verification of their demographic data identified them as brothers. After confirmation of their paternity affiliation using the AmpFlSTR(®)YFiler™ (Applied Biosystems), we used two other multiplexes kits to improve the differentiation rate. The PowerPlex(®) ESX17 System (Promega) added one locus, SE33, who exhibits four different alleles. The second kit, the AmpFlSTR(®)MiniFiler™ (Applied Biosystems) added three more loci. Only one allele difference was found. In order to increase the discrimination power between related and unrelated individuals, we recommend that the DNA laboratories consider using a larger multiplex typing kit in cases like the one informed here.
本报告展示了涉及兄弟姐妹时 DNA 鉴定的局限性。以色列 DNA 数据库通常使用 PowerPlex(®) ESI16 系统（Promega）对嫌疑人样本进行扩增。在将一系列嫌疑人上传到数据库软件时，我们发现两名嫌疑人之间存在异常高数量的共享等位基因，共有 32 个等位基因中有 31 个。通过验证他们的人口统计学数据，确定他们是兄弟。在使用 AmpFlSTR(®)YFiler™（Applied Biosystems）确认他们的亲子关系后，我们使用另外两个多重试剂盒来提高区分率。PowerPlex(®) ESX17 系统（Promega）增加了一个 SE33 位点，该位点有四个不同的等位基因。第二个试剂盒 AmpFlSTR(®)MiniFiler™（Applied Biosystems）增加了三个更多的位点。只发现了一个等位基因差异。为了提高相关和不相关个体之间的区分能力，我们建议 DNA 实验室在像本文所报告的案例中考虑使用更大的多重分型试剂盒。
