Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Ultrason Sonochem. 2014 Jan;21(1):310-6. doi: 10.1016/j.ultsonch.2013.07.014. Epub 2013 Aug 1.
Ultrasound-mediated drug delivery was established using liposomes that were modified with the thermosensitive polymer (TSP) poly(NIPMAM-co-NIPAM), which sensitized the liposomes to high temperatures. TSP-modified liposomes (TSP liposomes) released encapsulated calcein under 1 MHz ultrasound irradiation at 0.5 W/cm(2) for 120 s as well as the case under incubation at 42 °C for 15 min. In addition, uptake of the drug released from TSP liposomes by cancer cells was enhanced by ultrasound irradiation. In a cell injury assay using doxorubicin (DOX)-loaded TSP liposomes and ultrasound irradiation, cell viability of HepG2 cells at 6 h after ultrasound irradiation (1 MHz, 0.5 W/cm(2) for 30 s) with DOX-loaded TSP liposomes (TSP/lipid ratio=1) was 60%, which was significantly lower than that of the control conditions such as DOX-loaded TSP liposomes alone and DOX-loaded intact liposomes under ultrasound irradiation.
超声介导的药物传递是利用经热敏聚合物（TSP）聚（NIPMAM-co-NIPAM）修饰的脂质体来实现的，该聚合物使脂质体对高温敏感。TSP 修饰的脂质体（TSP 脂质体）在 1 MHz 超声辐射下以 0.5 W/cm(2)的功率照射 120 s 以及在 42°C 孵育 15 min 的情况下释放包裹的钙黄绿素。此外，超声辐射增强了癌细胞对从 TSP 脂质体中释放的药物的摄取。在使用载多柔比星（DOX）的 TSP 脂质体和超声辐射的细胞损伤测定中，在超声辐射（1 MHz，0.5 W/cm(2)，30 s）后 6 小时，HepG2 细胞的存活率为 60%，与单独载 DOX 的 TSP 脂质体和超声辐射下载 DOX 的完整脂质体等对照条件相比，显著降低。
