Laboratory for Conservation and Utilization of Bio-Resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan, 650091, China.
Appl Microbiol Biotechnol. 2013 Oct;97(19):8683-92. doi: 10.1007/s00253-013-5178-1. Epub 2013 Aug 16.
The nematode-trapping fungus Arthrobotrys oligospora is the best-studied fungus for understanding the interaction between fungi and nematodes. The fungus uses three-dimensional adhesive networks to capture nematodes and then penetrates into the worms through their cuticle. Here we examine the effects of fungal cell wall related proteins on morphogenesis and virulence of the fungi. We focused on the changes in its proteomic and transcriptional profiles during its transition from saprophytic to predatory phase. Isobaric tags for relative and absolute quantitation (iTRAQ) proteomics using the liquid chromatography/mass spectrometry (LC/MS) method revealed an extended set of virulence related proteins, such as adhesins and serine proteases, on the cell wall of A. oligospora. Transcription analyses of their coding genes revealed an important set of candidate virulence factors. Our analyses also show that glycosyl hydrolases likely play important roles in trap formation of A. oligospora. The adhesins on the three-dimensional adhesive networks may have two functions: to enable the mycelia to stick to nematodes and to serve as important constituents of the extracellular matrix that harbors many secreted virulence related proteins. This study is the first to systematically identify cell wall related proteins that are important in the trap formation and infection of the fungus against nematode hosts.
食线虫真菌节丛孢菌是研究真菌与线虫相互作用的最佳模式生物。该真菌利用三维粘性网络捕获线虫，然后穿透线虫的角质层进入线虫体内。在这里，我们研究了与真菌细胞壁相关的蛋白质对真菌形态发生和毒力的影响。我们专注于真菌从腐生到捕食阶段转变过程中其蛋白质组和转录组图谱的变化。采用液相色谱/质谱 (LC/MS) 方法的等重同位素标记相对和绝对定量 (iTRAQ) 蛋白质组学揭示了一套扩展的与毒力相关的蛋白质，如细胞壁上的黏附素和丝氨酸蛋白酶。对其编码基因的转录分析揭示了一组重要的候选毒力因子。我们的分析还表明，糖苷水解酶可能在节丛孢菌的诱捕形成中发挥重要作用。三维粘性网络上的黏附素可能具有两个功能：使菌丝黏附在线虫上，并作为含有许多分泌的与毒力相关的蛋白质的细胞外基质的重要组成部分。这项研究首次系统地鉴定了在真菌对线虫宿主的诱捕形成和感染过程中起重要作用的与细胞壁相关的蛋白质。
