Liang Lianming, Gao Hong, Li Jinzhou, Liu Lu, Liu Zhiheng, Zhang Ke-Qin
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, Yunnan, China.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, Yunnan, China.
Fungal Biol. 2017 Jan;121(1):11-20. doi: 10.1016/j.funbio.2016.08.010. Epub 2016 Aug 29.
The Woronin body is a unique organelle in Pezizomycotina species. Following the injury of hyphae, it can quickly seal the septal pores to reduce the loss of cytoplasm and promote hyphal healing. The Woronin body is also considered a significant factor in efficient pathogenesis in many fungal pathogens. In this study, we identified AoHex1, a homologue of Neurospora crassa Hex1, a main component of the Woronin body in the genome of the nematode-trapping fungus Arthrobotrys oligospora. To study the biological function of the AoHex1 gene, the gene was deleted and its phenotypes assessed. Inactivation of this gene led to the loss of the Woronin body. The ΔAoHex1 strain showed compromised growth rate, conidiation, and anti-stress abilities. Moreover, trap formation was completely absent in the mutant strain, which could no longer capture nematodes. Our results suggest that the Woronin body plays an important role in growth, conidiation, anti-stress, trap formation, and virulence against nematodes in A. oligospora.
沃罗宁体是粪壳菌纲物种中一种独特的细胞器。在菌丝受伤后，它能迅速封闭隔膜孔以减少细胞质流失并促进菌丝愈合。沃罗宁体也被认为是许多真菌病原体高效致病的一个重要因素。在本研究中，我们在捕食线虫真菌少孢节丛孢菌的基因组中鉴定出AoHex1，它是粗糙脉孢菌Hex1的同源物，而Hex1是沃罗宁体的主要成分。为研究AoHex1基因的生物学功能，我们对该基因进行了缺失并评估其表型。该基因的失活导致沃罗宁体缺失。ΔAoHex1菌株的生长速率、分生孢子形成及抗逆能力均受损。此外，突变菌株中完全没有捕虫器形成，无法再捕获线虫。我们的结果表明，沃罗宁体在少孢节丛孢菌的生长、分生孢子形成、抗逆、捕虫器形成及对线虫的毒力方面发挥重要作用。
