Wu Qin-Yi, Zhu Yue-Yan, Zou Cheng-Gang, Kang Ying-Qian, Liang Lian-Ming
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P. R. China.
Department of Microbiology, Guizhou Medical University, Guiyang, 550004, P. R. China.
J Microbiol. 2016 Nov;54(11):768-773. doi: 10.1007/s12275-016-6272-8. Epub 2016 Oct 29.
Turgor is very important for the invasive growth of fungal pathogens. Glycerol, a highly osmotic solvent, is considered to play an important role in turgor generation. The nematophagous fungus Arthrobotrys oligospora mainly lives as a saprophyte. In the presence of nematodes, A. oligospora enters the parasitic stage by forming three-dimensional networks (traps) to capture nematodes. In A. oligospora, we found that glycerol accumulated during nematode-induced trap formation. We demonstrated that deleting gph1, which encodes glycogen phosphorylase, decreased the glycerol content, compared with that of a wild-type strain. Although the number of traps induced by nematodes was not affected in the Δgph1 mutant, the capture rate was lower. Meanwhile, deleting gph1 also affected the growth rate and conidiation capacity of the fungus. These results indicate that glycerol derived from GPH1 is essential for the full virulence of A. oligospora against nematodes.
膨压对于真菌病原体的侵入性生长非常重要。甘油是一种高渗溶剂，被认为在膨压产生中起重要作用。捕食线虫真菌少孢节丛孢主要以腐生菌的形式生存。在有线虫存在的情况下，少孢节丛孢通过形成三维网络（陷阱）进入寄生阶段以捕获线虫。在少孢节丛孢中，我们发现在线虫诱导陷阱形成过程中甘油会积累。我们证明，与野生型菌株相比，缺失编码糖原磷酸化酶的gph1会降低甘油含量。尽管线虫诱导的陷阱数量在Δgph1突变体中不受影响，但捕获率较低。同时，缺失gph1也影响了真菌的生长速率和产孢能力。这些结果表明，来源于GPH1的甘油对于少孢节丛孢对线虫的完全毒力至关重要。
