Vattanaviboon P, Riddhimat R, Sirisali K, Lebnark T
Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
J Med Assoc Thai. 1990 Apr;73(4):202-5.
Thirty-two unconcentrated urines were electrophoresed on cellogels in 0.05 M barbital buffer, pH 8.6, at 280 volts for 35 minutes. Instead of concentrating 100 fold, the urine samples were directly applied on the gel in volumes from 1.2 to 3.6 microliters and Coomassie dye was used for staining. This makes it possible to observe the protein patterns at trace concentration by reagent strips (about 0.05-0.2 g/l). This method is simple, convenient and suited for routine services.
32份未浓缩的尿液在0.05M巴比妥缓冲液（pH 8.6）中，于280伏电压下在纤维素凝胶上进行电泳35分钟。尿液样本未进行100倍浓缩，而是直接以1.2至3.6微升的体积加样到凝胶上，并用考马斯染料进行染色。这使得通过试剂条能够观察到痕量浓度的蛋白质图谱（约0.05 - 0.2g/l）。该方法简单、方便，适用于常规检测。
