Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, 230036, China.
In Vitro Cell Dev Biol Anim. 2014 Jan;50(1):75-86. doi: 10.1007/s11626-013-9673-6. Epub 2013 Aug 16.
Adipose-derived stem cells (ADSCs), a subset of mesenchymal stem cells, have promising potential for regenerative medicine applications. However, the efficient culture of mouse adipose-derived stem cells (mADSCs) is complicated or impracticable and many properties of mADSCs are still unknown. Here, we report that vitamin C (Vc) is available for the long-term culture of mADSCs in vitro. Compared with that cultured without Vc, mADSCs growing in Vc-added media proliferate faster. The occurrence of replicative senescence and transformation of Vc-treated mADSCs is also postponed. Vc also enhanced the secretory activity of collagen and adipogenic differentiation ability of mADSCs. Moreover, the expression of CD44, Sca-1, and CD105 is higher in Vc-treated mADSCs than nontreated ones. We also found that genes related to proliferation, secretion, and pluripotency are all promoted in Vc-treated mADSCs. However, the adipogenesis ability and expression of CD44, Sca-1, and CD105 decreased when passage increased from very low passages, in parallel to the downregulation of closed-related gene expression. Our results indicate that Vc is essential for the maintenance of original properties of mADSCs in vitro; additional insights into the function of Vc on mADSCs are provided. Furthermore, as the passage increased in six passages, the characteristics of mADSCs with Vc addition were also revealed.
脂肪来源干细胞（ADSCs），间充质干细胞的一个亚群，在再生医学应用中具有很大的潜力。然而，高效培养小鼠脂肪来源干细胞（mADSCs）很复杂或不切实际，并且 mADSCs 的许多特性仍不清楚。在这里，我们报告维生素 C（Vc）可用于 mADSCs 的长期体外培养。与不添加 Vc 的培养基相比，在添加 Vc 的培养基中生长的 mADSCs 增殖更快。Vc 处理的 mADSCs 发生复制性衰老和转化的几率也被推迟。Vc 还增强了 mADSCs 的胶原分泌活性和脂肪生成分化能力。此外，在 Vc 处理的 mADSCs 中的 CD44、Sca-1 和 CD105 的表达水平高于未处理的 mADSCs。我们还发现，与增殖、分泌和多能性相关的基因在 Vc 处理的 mADSCs 中均被促进。然而，当传代从极低代增加时，脂肪生成能力和 CD44、Sca-1 和 CD105 的表达下降，同时相关基因表达下调。我们的结果表明，Vc 对于维持 mADSCs 的原始特性是必需的；进一步深入研究了 Vc 对 mADSCs 的作用。此外，随着传代增加到六代，添加 Vc 的 mADSCs 的特性也被揭示。
