Arezoumand Khatereh Saei, Alizadeh Effat, Esmaeillou Mohammad, Ghasemi Maryam, Alipour Shahriar, Pilehvar-Soltanahmadi Younes, Zarghami Nosratollah
The Umbilical Cord Stem Cell Research Center (UCSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
In Vitro Cell Dev Biol Anim. 2018 Mar;54(3):205-216. doi: 10.1007/s11626-018-0228-8. Epub 2018 Jan 29.
In recent decades, mesenchymal stem cells originated from adipose tissue (adipose-derived stem cells, ASCs) have gained increased attention for production of cell-based therapeutics. Emu oil as a natural compound showed antioxidant effects in previous studies. The goal of this study was to investigate the effect of crude emu oil on the proliferation, cell cycle progression, stemness genes expression, and in vitro wound healing potential of ASCs. An emulsion of emu oil was prepared using egg lecithin and butylated hydroxytoluene to improve bioavailability and solubility of emu oil in the expansion medium. The ASCs were treated using a series of emu oil concentrations in emulsion form, diluted in expansion medium (0.03-3 mg/ml). The emu oil-free emulsion was used as control treatment. The results revealed that emu oil (1.25 mg/ml) in emulsion form significantly (p < 0.001) increased ASCs proliferation and colony formation. Additionally, emu oil caused upregulation of stemness marker genes (Sox2, Oct4, Nanog, and Nestin) (p < 0.05). The cell cycle analysis after emu oil treatments showed an increase in the population of ASCs in S-phase of the cell cycle. Besides, an accelerated in vitro scratch wound healing was observed in emu oil-treated ASCs. Emu oil enhanced proliferation, colony formation, stemness genes expression, and in vitro wound healing of ASCs. These findings suggest that emu oil treatment could maintain the stemness of ex vivo cultivated ASCs and enhance their regenerative potential.
近几十年来，源自脂肪组织的间充质干细胞（脂肪来源干细胞，ASCs）在基于细胞的治疗产品生产中受到了越来越多的关注。在先前的研究中，鸸鹋油作为一种天然化合物显示出抗氧化作用。本研究的目的是探讨粗制鸸鹋油对ASCs增殖、细胞周期进程、干性基因表达及体外伤口愈合潜力的影响。使用卵磷脂和丁基化羟基甲苯制备了鸸鹋油乳液，以提高鸸鹋油在扩增培养基中的生物利用度和溶解度。将ASCs用一系列乳液形式的鸸鹋油浓度处理，在扩增培养基中稀释（0.03 - 3mg/ml）。不含鸸鹋油的乳液用作对照处理。结果显示，乳液形式的鸸鹋油（1.25mg/ml）显著（p < 0.001）增加了ASCs的增殖和集落形成。此外，鸸鹋油导致干性标记基因（Sox2、Oct4、Nanog和Nestin）上调（p < 0.05）。鸸鹋油处理后的细胞周期分析显示细胞周期S期的ASCs群体增加。此外，在鸸鹋油处理的ASCs中观察到体外划痕伤口愈合加速。鸸鹋油增强了ASCs的增殖、集落形成、干性基因表达和体外伤口愈合。这些发现表明，鸸鹋油处理可以维持体外培养的ASCs的干性并增强其再生潜力。
