Department of Botany, Utkal University , Vanivihar, Bhubaneswar -751004, Orissa , India.
Braz J Microbiol. 2012 Oct;43(4):1499-507. doi: 10.1590/S1517-838220120004000034. Epub 2012 Jun 1.
Bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, Zymomonas mobilis MTCC 92 entrapped in luffa (Luffa cylindrica L.) sponge discs and Ca-alginate gel beads as the immobilizing matrices. At the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and Ca-alginate entrapped Z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. There was no statistical significant difference (Fischer's LSD) in sugar utilization (t = 0.254, p<0.801) and ethanol production (t =-0.663, p<0.513) between the two immobilization matrices used. Further, the immobilized cells in both the matrices were physiologically active for three more cycles of operation with less than 15 % decrease in ethanol yield in the 4(th) cycle, which was due to some leakage of cells. In conclusion, luffa sponge was found to be equally good as Ca-alginate as a carrier material for bacterial (Z. mobilis) cell immobilization for ethanol production. Further, it has added advantages such as it is cheap, non-corrosive and has no environmental hazard.
采用丝瓜（Luffa cylindrica L.）海绵片和 Ca-海藻酸钠凝胶珠作为固定化基质，研究了用细菌运动发酵单胞菌（Zymomonas mobilis MTCC 92）从甘蔗废糖蜜（稀释至 15%糖 w/v）中生产生物乙醇。在 96 h 发酵结束时，丝瓜和 Ca-海藻酸钠包埋 Z. mobilis 细胞的最终乙醇浓度分别为 58.7±0.09 和 59.1±0.08 g/l 废糖蜜，分别显示出 83.25±0.03 和 84.6±0.02%的糖转化率。两种固定化基质的糖利用率（t=0.254，p<0.801）和乙醇产量（t=-0.663，p<0.513）没有统计学显著差异（Fischer's LSD）。此外，在第 4 个周期中，由于细胞泄漏，两种基质中的固定化细胞的乙醇产率下降不到 15%，仍保持着生理活性，可再进行三个周期的操作。总之，丝瓜海绵作为细菌（运动发酵单胞菌）细胞固定化生产乙醇的载体材料与 Ca-海藻酸钠一样好。此外，它还具有价格低廉、无腐蚀性、无环境危害等优点。
