Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences; Graduate University of Chinese Academy of Sciences, Beijing.
Insect Sci. 2013 Oct;20(5):643-54. doi: 10.1111/j.1744-7917.2012.01565.x. Epub 2012 Dec 6.
The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and has since spread across 2.77 × 10(5) km(2) in Xinjiang Uygur Autonomous Region. To better understand genetic variation and migration patterns, we used seven polymorphic microsatellite loci to elucidate the genetic relationships and gene flow among 10 CPB populations across Xinjiang. (i) Overall low levels of genetic diversity were detected on the entire population in Xinjiang but most of the diversity was retained among populations during invasion. (ii) The mean pairwise FST was low (0.071 ± 0.043) among populations. The genetic differentiation was little (pairwise FST 0.038 ± 0.016) between the five interior populations (Wusu, Urumqi, Jimsar, Qitai and Mulei) and Tacheng population. The six populations might come from the same genetic group via Bayesian clustering and were closely related on a neighbor-joining tree. Combining the history data, the five interior populations may have originated from Tacheng. (iii) Gene flow was frequent, especially among the five interior populations. Individuals from the interior populations could be assigned to Tacheng at higher probabilities (means 0.518 ± 0.127) than vice versa (means 0.328 ± 0.074), suggesting that the beetle population has spread from the border to the interior in Xinjiang.
科罗拉多马铃薯甲虫（CPB），Leptinotarsa decemlineata（Say），是一种在全球范围内具有侵略性的著名入侵物种，它会攻击马铃薯和其他茄科作物。CPB 于 1993 年首次在中国发现，此后已在新疆维吾尔自治区蔓延了 2.77×10(5)km(2)。为了更好地了解遗传变异和迁移模式，我们使用了七个多态性微卫星位点来阐明新疆 10 个 CPB 种群的遗传关系和基因流。（i）在整个新疆种群中检测到总体遗传多样性水平较低，但在入侵过程中大部分多样性都保留在种群之间。（ii）种群间平均配对 FST 较低（0.071±0.043）。遗传分化很小（0.038±0.016），五个内陆种群（乌苏、乌鲁木齐、吉木萨尔、奇台和木垒）和塔城种群之间。这六个种群可能来自同一遗传群体，通过贝叶斯聚类和在邻接树上密切相关。结合历史数据，五个内陆种群可能起源于塔城。（iii）基因流频繁，尤其是在五个内陆种群之间。内陆种群的个体被分配到塔城的可能性更高（平均值 0.518±0.127），而反之亦然（平均值 0.328±0.074），表明甲虫种群已从边境向新疆内陆扩散。
