Yu Yanxue, Wang Yuhan, Li Hongwei, Yu Xin, Shi Wangpeng, Zhai Junfeng
Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China.
Biowavelet Ltd., Chongqing, China.
Front Microbiol. 2021 Mar 19;12:639913. doi: 10.3389/fmicb.2021.639913. eCollection 2021.
Microbial communities in insects are related to their geographical sources and contribute to adaptation to the local habitat. The Colorado potato beetle () (CPB) is a potato pest that causes serious economic losses in Xinjiang Uygur Autonomous Region (XJ) and Heilongjiang Province (HL), China. The influence of microorganisms in the invasion and dispersal of CPB is unclear. We studied microbial communities of CPB collected from nine geographic sources in China using high throughput sequencing technology. Bacteroidetes, Firmicutes, and Proteobacteria were the most dominant phyla, Clostridia, Bacteroidetes, and γ-Proteobacteria were the most dominant classes, Enterobacterales, Lactobacillales, Clostridiales, and Bacteroidales were the most dominant orders, and Enterobacteriaceae, Streptococcidae, Verrucomicrobiaceae, and Rikenellaceae were the most dominant families. There were significant differences, among sources, in the relative abundance of taxa at the genus level. A total of 383 genera were identified, and the dominant bacteria at the genus level were compared between XJ and HL. was the unique dominant microorganism in the HL area, and the other four microorganisms (, , , and ) were common within the 2 regions. Bacterial community diversity in CPB from Urumqi, Jimunai, and Wenquan was higher than diversity in other regions. T-Distributed Stochastic Neighbor Embedding (tSNE) analysis indicated that order and genus were appropriate taxonomic levels to distinguish geographical sources of CPB. These findings provide insight into the diversity of microorganisms of CPB in the differences among geographically isolated populations.
昆虫体内的微生物群落与其地理来源相关，并有助于适应当地栖息地。马铃薯甲虫是一种马铃薯害虫，在中国新疆维吾尔自治区和黑龙江省造成了严重的经济损失。微生物在马铃薯甲虫入侵和扩散中的作用尚不清楚。我们使用高通量测序技术研究了从中国九个地理来源采集的马铃薯甲虫的微生物群落。拟杆菌门、厚壁菌门和变形菌门是最主要的门类，梭菌纲、拟杆菌纲和γ-变形菌纲是最主要的纲，肠杆菌目、乳杆菌目、梭菌目和拟杆菌目是最主要的目，肠杆菌科、链球菌科、疣微菌科和理研菌科是最主要的科。在属水平上，不同来源的分类单元相对丰度存在显著差异。共鉴定出383个属，并比较了新疆和黑龙江地区属水平上的优势细菌。是黑龙江地区独特的优势微生物，其他四种微生物（、、和）在两个地区都很常见。来自乌鲁木齐、吉木乃和温泉的马铃薯甲虫的细菌群落多样性高于其他地区。T分布随机邻域嵌入（tSNE）分析表明，目和属是区分马铃薯甲虫地理来源的合适分类水平。这些发现为了解地理隔离种群差异中马铃薯甲虫微生物的多样性提供了见解。
