Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China.
J Econ Entomol. 2010 Aug;103(4):1365-71. doi: 10.1603/ec10031.
Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), has become the economically most important insect defoliator of potatoes, Solanum tuberosum L., in northern Xinjiang Uygur autonomous region in China. Currently, control of Colorado potato beetle relies mainly on chemical insecticides. And this may result in insecticide resistance. In this study, LD50 values were measured by a topical bioassay for 14 conventional insecticides in seven local populations from Urumqi, Changji, Tacheng, Nilka, Gongliu, Qapqal, and Tekes counties (cities). The Tekes field population was the most susceptible population and was selected as a reference strain. Compared with the Tekes strain, the Changji, Qapqal, Nilka, Tacheng, and Gongliu populations exhibited moderate to very high levels of resistance to cyhalothrin. The Qapqal and Changji populations showed a moderate and a very high resistance to deltamethrin, respectively. And the Changji population developed a high resistance against alpha-cypermethrin. Moreover, the Qapqal population had a moderate resistance to carbofuran, and the Urumqi population reached high level of resistance to endosulfan. Possible resistance mechanisms of the Changji and Qapqal populations were determined using three enzyme inhibitors. Triphenyl phosphate (TPP), diethylmeleate, and piperonyl butoxide (PBO) had little synergism to cyhalothrin in the two populations. In contrast, PBO and TPP exhibited some synergistic effects to carbofuran in the Qapqal population, indicating the involvement of monooxygenases and esterases in conferring carbofuran resistance. It seems that additional mechanisms, such as target site insensitivity, should play an important role in Colorado potato beetle resistances to cyhalothrin and carbofuran in northern Xinjiang local populations.
马铃薯甲虫，Leptinotarsa decemlineata（Say）（鞘翅目：叶甲科），已成为中国新疆北部地区马铃薯（Solanum tuberosum L.）经济上最重要的昆虫食叶害虫。目前，对马铃薯甲虫的防治主要依赖于化学杀虫剂。这可能导致抗药性。在这项研究中，通过对乌鲁木齐、昌吉、塔城、尼勒克、巩留、且末和特克斯县（市）的七个地方种群进行的体表生物测定法测量了 14 种常规杀虫剂的 LD50 值。特克斯田间种群是最敏感的种群，被选为参考品系。与特克斯品系相比，昌吉、且末、尼勒克、塔城和巩留种群对氯氟氰菊酯表现出中等至高水平的抗性。且末种群对溴氰菊酯表现出中度至高度抗性，昌吉种群对高效氯氰菊酯表现出高度抗性。此外，且末种群对甲氰菊酯具有中度抗性，而乌鲁木齐种群对硫丹具有高抗药性。使用三种酶抑制剂测定了昌吉和且末种群的可能抗性机制。三苯基磷酸酯（TPP）、二乙基甲醚和胡椒基丁醚（PBO）对两种种群的氯氟氰菊酯几乎没有增效作用。相比之下，PBO 和 TPP 对且末种群的克百威表现出一定的增效作用，表明单加氧酶和酯酶参与了克百威的抗性。似乎目标部位不敏感性等其他机制应该在新疆北部地方种群对氯氟氰菊酯和克百威的马铃薯甲虫抗性中起重要作用。
