不同热处理方式对杏鲍菇提取物中酚类物质组成与抗氧化活性的影响。
Effects of various heat treatments on phenolic profiles and antioxidant activities of Pleurotus eryngii extracts.
机构信息
Food and Nutritional Science, School of Biological Science, The Univ. of Hong Kong, Pokfulam Rd., Hong Kong.
出版信息
J Food Sci. 2013 Aug;78(8):C1122-9. doi: 10.1111/1750-3841.12189.
Free (FP) and bound phenolics (BP) were extracted from freeze dried (FD), oven dried (OD), as well as boiling treated (BT) Pleurotus eryngii samples. Free, bound, total phenolics were quantified using Folin-Ciocalteau assay. Qualitative and quantitative analysis of phenolic compounds were carried out using high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD), and a total of 8 phenolic compounds were detected. Free phenolic contents followed the order: Freeze-dried free phenolics (FDFP) > Oven-dried free phenolics (ODFP) > Boiling-treated free phenolics (BTFP), and ranged from 95.42 (BTFP) to 442.50 (ODPF) μg gallic acid equivalents (GAE)/g dry weight (DW). Bound and total phenolic contents followed the order: FD > OD > BT, and ranged from 218.33 (BTBP) to 774.17 (FDBP) and 313.75 (BT total phenolics) to 1090.42 (FD total phenolics) μg GAE/g DW. Bound phenolics contributed 49.76% (OD), 69.59% (BT), and 71% (FD) of the total phenolic contents. All free and bound phenolic extracts were investigated for their antioxidant activities by 3 different assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide anion radical scavenging activity. FDFP showed strongest DPPH radical scavenging activity (IC50 at 32.61 μg/mL), ODFP showed strongest reducing power (IC50 at 26.31 μg/mL), and BTBP showed strongest superoxide anion radical scavenging activity (IC50 at 14.07 μg/mL).
从冷冻干燥（FD）、烘箱干燥（OD）和煮沸处理（BT）的杏鲍菇样品中提取游离酚（FP）和结合酚（BP）。使用福林-肖卡（Folin-Ciocalteau）法测定游离、结合和总酚含量。采用高效液相色谱（HPLC）-二极管阵列检测器（DAD）对酚类化合物进行定性和定量分析，共检测到 8 种酚类化合物。游离酚含量顺序为：冷冻干燥游离酚（FDFP）>烘箱干燥游离酚（ODFP）>煮沸处理游离酚（BTFP），范围为 95.42（BTFP）至 442.50（ODPF）μg 没食子酸当量（GAE）/g 干重（DW）。结合酚和总酚含量顺序为：FD>OD>BT，范围为 218.33（BTBP）至 774.17（FDBP）和 313.75（BT 总酚）至 1090.42（FD 总酚）μg GAE/g DW。结合酚分别占总酚含量的 49.76%（OD）、69.59%（BT）和 71%（FD）。通过 3 种不同的测定方法，即 1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、还原能力和超氧阴离子自由基清除活性，研究了所有游离和结合酚提取物的抗氧化活性。FDFP 表现出最强的 DPPH 自由基清除活性（IC50 为 32.61μg/mL），ODFP 表现出最强的还原能力（IC50 为 26.31μg/mL），BTBP 表现出最强的超氧阴离子自由基清除活性（IC50 为 14.07μg/mL）。
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