Faculty of Pharmacy, The University of Sydney, NSW, Australia.
J Pharm Pharm Sci. 2013;16(2):304-12. doi: 10.18433/j34g7p.
The aim of this study was to investigate the mechanism of (S)-[6]-gingerol in promoting glucose uptake in L6 skeletal muscle cells.
The effect of (S)-[6]-gingerol on glucose uptake in L6 myotubes was examined using 2-[1,2-3H]-deoxy-D-glucose. Intracellular Ca2+ concentration was measured using Fluo-4. Phosphorylation of AMPKα was determined by Western blotting analysis.
(S)-[6]-Gingerol time-dependently enhanced glucose uptake in L6 myotubes. (S)-[6]-Gingerol elevated intracellular Ca2+ concentration and subsequently induced a dose- and time-dependent enhancement of threonine172 phosphorylated AMPKα in L6 myotubes via modulation by Ca2+/calmodulin-dependent protein kinase kinase.
The results indicated that (S)-[6]-gingerol increased glucose uptake in L6 skeletal muscle cells by activating AMPK. (S)-[6]-gingerol, a major component of Zingiber officinale, may have potential for development as an antidiabetic agent.
本研究旨在探讨（S）-[6]-姜烯酚促进 L6 骨骼肌细胞葡萄糖摄取的机制。
采用 2-[1,2-3H]-脱氧-D-葡萄糖检测（S）-[6]-姜烯酚对 L6 肌管葡萄糖摄取的影响。使用 Fluo-4 测定细胞内 Ca2+浓度。通过 Western 印迹分析测定 AMPKα 的磷酸化。
（S）-[6]-姜烯酚可时间依赖性地增强 L6 肌管的葡萄糖摄取。（S）-[6]-姜烯酚通过调节 Ca2+/钙调蛋白依赖性蛋白激酶激酶，增加细胞内 Ca2+浓度，并随后诱导 L6 肌管中苏氨酸 172 磷酸化 AMPKα 的剂量和时间依赖性增强。
结果表明，（S）-[6]-姜烯酚通过激活 AMPK 增加 L6 骨骼肌细胞的葡萄糖摄取。（S）-[6]-姜烯酚是生姜的主要成分之一，可能具有开发为抗糖尿病药物的潜力。
