Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile.
Biol Res. 2013;46(2):177-82. doi: 10.4067/S0716-97602013000200009.
Quillaja saponaria Mol. (Quillajaceae) is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in the central zone, and compare the composition of the chromatographic profiles of nectar and honey to known phenolic compounds. The results obtained by HPLC-DAD (high-performance liquid chromatography with diode-array detection) showed a similar profile of phenolic compounds, in which gallic acid, myricetin, rutin, quercetin and naringenin were identified. The phenolic compounds detected could be used as a reference for future studies for determining potential chemical markers of this honey, complementing the present identification of honeys by determining their botanical origin. The identification of bioindicators of the floral origins for honey of this species could provide added value to honey commercialization by certifying the botanical origin of their chemical features and biological attributes.
荆树皮(Quillajaceae)是智利最重要的产蜜物种之一,主要作为单花蜜源。由 A. mellifera 酿造的蜂蜜具有抗病原体的生物活性和与酚类化合物有关的抗氧化能力,这些酚类化合物来自花蜜,是蜜蜂采蜜的结果。本研究的目的是鉴定和定量荆树皮花蜜和养蜂场蜂蜜中的酚类化合物,并比较花蜜和蜂蜜的色谱图谱组成与已知酚类化合物的相似性。通过 HPLC-DAD(高效液相色谱-二极管阵列检测)获得的结果表明,酚类化合物具有相似的图谱,其中鉴定出了没食子酸、杨梅素、芦丁、槲皮素和柚皮素。检测到的酚类化合物可以作为未来研究的参考,以确定这种蜂蜜的潜在化学标志物,补充通过确定其植物来源来确定蜂蜜的现有鉴定。确定这种蜂蜜的花源生物标志物可以通过证明其化学特征和生物属性的植物来源来为蜂蜜的商业化提供附加值。