Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
Saudi Pharm J. 2012 Jul;20(3):249-53. doi: 10.1016/j.jsps.2012.03.004. Epub 2012 Mar 31.
An innovative spectrophotometric method was developed for simultaneous determination of compounds with interfering spectra in binary mixtures without previous separation, showing significant advantages over the conventional methods regarding minimal data manipulation and applicability. The proposed method was applied for the determination of retinoic acid and hydroquinone in laboratory-prepared mixtures with mean percentage recoveries 100.13 ± 0.31 and 99.99 ± 0.04, respectively, and in their pharmaceutical formulation with mean percentage recoveries 100.13 ± 0.86 and 100.07 ± 0.58, respectively. The method was validated according to USP guidelines and can be applied for routine quality control testing.
建立了一种无需预先分离即可同时测定二元混合物中具有光谱干扰化合物的分光光度新方法，与常规方法相比，该方法在数据处理和适用性方面具有显著优势。该方法用于测定实验室制备的混合物中的视黄酸和对苯二酚，平均回收率分别为 100.13 ± 0.31%和 99.99 ± 0.04%，在其药物制剂中的平均回收率分别为 100.13 ± 0.86%和 100.07 ± 0.58%。该方法符合 USP 指南，可以用于常规质量控制检测。
