Bonazzi D, Gotti R, Andrisano V, Cavrini V
Dipartimento di Scienze Farmaceutiche, Bologna, Italy.
J Pharm Biomed Anal. 1997 Nov;16(3):431-8. doi: 10.1016/s0731-7085(97)00075-7.
Derivative UV spectroscopy and high performance liquid chromatography (HPLC) were applied to the determination of angiotensin-converting enzyme (ACE) inhibitors in their pharmaceutical dosage forms. For spectrophotometric determinations, the more appropriate derivative order was selected for each drug: ramipril (third-order), benazepril (second-order), enalapril maleate (second-order), lisinopril (first- and second-order) and quinapril (first-order). Reverse phase HPLC procedures (ODS column) were developed able to provide a single, symmetric peak for each drug; mixtures A-B, where A is 20 mM sodium heptansulphonate (pH 2.5) and B is acetonitrile-THF (95:5 v/v), proved to be suitable mobile phases to obtain selective separations of the cited ACE inhibitors. At ambient temperature, a low pH value (2.5) was found to be critical to avoid peak splitting and band broadening.
采用导数紫外光谱法和高效液相色谱法（HPLC）测定药物剂型中的血管紧张素转换酶（ACE）抑制剂。对于分光光度法测定，为每种药物选择了更合适的导数阶数：雷米普利（三阶）、贝那普利（二阶）、马来酸依那普利（二阶）、赖诺普利（一阶和二阶）和喹那普利（一阶）。开发了反相HPLC程序（ODS柱），能够为每种药物提供单一的对称峰；混合物A - B，其中A为20 mM庚烷磺酸钠（pH 2.5），B为乙腈 - 四氢呋喃（95:5 v/v），被证明是获得上述ACE抑制剂选择性分离的合适流动相。在环境温度下，发现低pH值（2.5）对于避免峰分裂和谱带展宽至关重要。
