1 Department of Bioengineering, University of California , Berkeley, California.
Tissue Eng Part A. 2014 Jan;20(1-2):285-93. doi: 10.1089/ten.TEA.2013.0060. Epub 2013 Oct 17.
Due to high incidence of vascular bypass procedures, an unmet need for suitable vessel replacements exists, especially for small-diameter (<6 mm) vascular grafts. Here, we developed a novel, bilayered, synthetic vascular graft of 1-mm diameter that consisted of a microfibrous luminal layer and a nanofibrous outer layer, which was tailored to possess the same mechanical property as native arteries. We then chemically modified the scaffold with mucin, a glycoprotein lubricant on the surface of epithelial tissues, by either passive adsorption or covalent bonding using the di-amino-poly(ethylene glycol) linker to microfibers. Under static and physiological flow conditions, conjugated mucin was more stable than adsorbed mucin on the surfaces. Mucin could slightly inhibit blood clotting, and mucin coating suppressed platelet adhesion on microfibrous scaffolds. In the rat common carotid artery anastomosis model, grafts with conjugated mucin, but not adsorbed mucin, exhibited excellent patency and higher cell infiltration into the graft walls. Mucin, which can be easily obtained from autologous sources, offers a novel method for improving the hemocompatibility and surface lubrication of vascular grafts and many other implants.
由于血管旁路手术的发病率很高，因此存在对合适血管替代品的未满足需求，特别是对于小直径（<6 毫米）血管移植物。在这里，我们开发了一种新颖的双层合成血管移植物，直径为 1 毫米，由微纤维内腔层和纳米纤维外层组成，其机械性能与天然动脉相同。然后，我们通过使用二氨基聚（乙二醇）连接物将粘蛋白（上皮组织表面的糖蛋白润滑剂）化学修饰到支架上，要么通过被动吸附，要么通过共价键合到微纤维上。在静态和生理流动条件下，与表面接枝的粘蛋白相比，吸附的粘蛋白更稳定。粘蛋白可轻微抑制血液凝固，并且粘蛋白涂层可抑制血小板在微纤维支架上的黏附。在大鼠颈总动脉吻合模型中，与接枝粘蛋白的移植物（而非吸附粘蛋白的移植物）表现出良好的通畅性和更高的细胞向移植物壁内的浸润。粘蛋白可以从自体来源中轻易获得，为改善血管移植物和许多其他植入物的血液相容性和表面润滑性提供了一种新方法。
