Kantele A
National Public Health Institute, Helsinki, Finland.
Vaccine. 1990 Aug;8(4):321-6. doi: 10.1016/0264-410x(90)90088-4.
The immune response to different dosage schedules of oral live Salmonella typhi Ty21a vaccines was studied by enumeration of specific antibody-secreting cells (ASC) in the peripheral blood believed to have been stimulated by the vaccine antigen on mucosal surfaces and to be on their way back to those sites for local antibody secretion. Four groups of subjects were vaccinated with either three (3 x S), two (2 x S) or one (1 x S) dose of a suspension-formulated vaccine, or with three doses of vaccine in enteric-coated capsules (3 x E). The ASC-responses were highest in group 3 x S, followed by 3 x E, 2 x S and 1 x S, in this order. These differences parallel differences in protection from disease as observed in field trails with these regimens. This assay might therefore be useful for presumptive assessment of the protective ability of new vaccines or vaccine regimens. It certainly can be used to measure the immunogenicity of an oral vaccine.
通过对周围血液中特定抗体分泌细胞（ASC）进行计数，研究了口服活伤寒沙门氏菌Ty21a疫苗不同剂量方案的免疫反应。这些抗体分泌细胞被认为是受到粘膜表面疫苗抗原刺激后，正返回这些部位进行局部抗体分泌。四组受试者分别接种了三剂（3xS）、两剂（2xS）或一剂（1xS）悬浮剂型疫苗，或三剂肠溶胶囊疫苗（3xE）。ASC反应在3xS组中最高，其次依次为3xE组、2xS组和1xS组。这些差异与这些方案在现场试验中观察到的疾病预防差异相似。因此，该检测方法可能有助于对新疫苗或疫苗方案的保护能力进行初步评估。它当然可用于测量口服疫苗的免疫原性。
