Obi T U, McCullough K C, Taylor W P
Institute for Animal Disease Research, Pirbright Laboratory, Woking Surrey, U.K.
Zentralbl Veterinarmed B. 1990 Jul;37(5):345-52. doi: 10.1111/j.1439-0450.1990.tb01068.x.
Hyperimmune sera were produced by serial inoculation of rabbits with Vero cell-adapted, sucrose gradient-purified Nigerian peste des petits ruminants virus (PPRV) isolate. Two antisera produced, neutralized the homologous PPRV but not the heterologous rinderpest Kabette "O" virus. The antisera gave strong precipitin lines with purified PPRV antigens and were used to detect PPRV and rinderpest virus antigens from ante-mortem secretions and post-mortem tissue homogenates from PPR and rinderpest virus infected goats and cattle by the agar gel precipitation tests (AGPT). The hyperimmune sera gave good titration curves with both purified Nigerian goat and the United Arab Emirate wildlife PPRV isolates in the indirect enzyme linked immunosorbent assay (ELISA). Results of indirect ELISA showed that although there were some cross reactions with the rinderpest, canine-distemper and measles viruses, at 1:100 dilution, the antisera would give a positive signal with only the homologous PPR virus.
通过用适应Vero细胞、经蔗糖梯度纯化的尼日利亚小反刍兽疫病毒（PPRV）分离株对家兔进行连续接种来制备高免血清。所产生的两种抗血清能中和同源PPRV，但不能中和异源牛瘟卡贝特“O”病毒。这些抗血清与纯化的PPRV抗原产生强沉淀线，并用于通过琼脂凝胶沉淀试验（AGPT）检测来自感染PPR和牛瘟病毒的山羊和牛的生前分泌物及死后组织匀浆中的PPRV和牛瘟病毒抗原。在间接酶联免疫吸附测定（ELISA）中，高免血清与纯化的尼日利亚山羊PPRV分离株和阿拉伯联合酋长国野生动物PPRV分离株均呈现出良好的滴定曲线。间接ELISA结果表明，尽管与牛瘟、犬瘟热和麻疹病毒存在一些交叉反应，但在1:100稀释度下，抗血清仅会对同源PPR病毒给出阳性信号。
