Mariner J C, House J A, Mebus C A, van den Ende M C
Tufts University School of Veterinary Medicine, Section of International Veterinary Medicine, North Grafton, Massachusetts 01536.
Res Vet Sci. 1993 Mar;54(2):212-6. doi: 10.1016/0034-5288(93)90059-o.
The thermostable Vero cell-adapted rinderpest vaccine was evaluated in terms of immunogenicity as a heterologous vaccine against peste des petits ruminants. A titration to establish the minimum immunising dose was performed in American mixed breed goats by vaccinating test subjects with dilutions of Vero cell-adapted rinderpest vaccine and then challenging 26 days later with virulent peste des petits ruminants virus. All animals were followed for virus neutralising antibodies against both rinderpest and peste des petits ruminants virus after vaccination and challenge. The antibody response to vaccination was primarily against rinderpest virus with very low levels of cross-reactivity to peste des petits ruminants virus. Following challenge, animals which possessed anti-rinderpest neutralising antibodies remained clinically normal but mounted strong anti-peste des petits ruminants virus neutralising antibody responses indicating that replication of challenge virus took place without the induction of illness. The 50 per cent minimum goat immunising dose was 3 tissue culture infectious doses 50 per cent (TCID50) as established by serological response and protection against challenge. The thermostable Vero cell-adapted rinderpest vaccine is a suitable immunogen for the protection of goats against peste des petits ruminants.
对热稳定的适应Vero细胞的牛瘟疫苗作为一种抗小反刍兽疫的异源疫苗的免疫原性进行了评估。通过用适应Vero细胞的牛瘟疫苗稀释液给受试动物接种，然后在26天后用强毒小反刍兽疫病毒攻击，对美国杂交山羊进行滴定以确定最低免疫剂量。在接种疫苗和攻击后，对所有动物进行针对牛瘟和小反刍兽疫病毒的病毒中和抗体监测。接种疫苗后的抗体反应主要针对牛瘟病毒，与小反刍兽疫病毒的交叉反应水平很低。攻击后，拥有抗牛瘟中和抗体的动物保持临床正常，但产生了强烈的抗小反刍兽疫病毒中和抗体反应，表明攻击病毒发生了复制但未引发疾病。根据血清学反应和对攻击的保护作用确定，山羊的50%最低免疫剂量为3个50%组织培养感染剂量（TCID50）。热稳定的适应Vero细胞的牛瘟疫苗是保护山羊免受小反刍兽疫侵害的合适免疫原。
