用琼脂凝胶沉淀试验和对流免疫电泳检测小反刍兽疫（PPR）病毒抗原。
The detection of peste des petits ruminants (PPR) virus antigen by agar gel precipitation test and counter-immunoelectrophoresis.
作者信息
Obi T U, Patrick D
出版信息
J Hyg (Lond). 1984 Dec;93(3):579-86. doi: 10.1017/s0022172400065165.
Abstract
The detectability of peste des petits ruminants (PPR) viral antigen in both ante-mortem secretions and necropsy samples from experimentally infected goats was investigated by both the agar gel precipitation test (AGPT) and counter-immunoelectrophoresis (CIE). Viral antigen was detected from 42.6% of the samples tested by the AGPT and 80.3% by CIE. The detection of viral antigen in a high proportion of the ocular and nasal secretions as well as the faeces and buccal scrapings, particularly from those collected within seven days of the onset of fever, by both techniques, would seem to obviate the need for lymph node biopsies or post-mortem samples in order to make a diagnosis of PPRV infection.
摘要
通过琼脂凝胶沉淀试验（AGPT）和对流免疫电泳（CIE），研究了实验感染山羊生前分泌物和尸检样本中绵羊痘和山羊痘（PPR）病毒抗原的可检测性。AGPT检测的样本中有42.6%检测到病毒抗原，CIE检测的样本中有80.3%检测到病毒抗原。两种技术在高比例的眼鼻分泌物、粪便和颊部刮片中检测到病毒抗原，特别是在发热开始后7天内采集的样本中，这似乎消除了为诊断PPRV感染而进行淋巴结活检或尸检样本的必要性。
相似文献
1
The detection of peste des petits ruminants (PPR) virus antigen by agar gel precipitation test and counter-immunoelectrophoresis.用琼脂凝胶沉淀试验和对流免疫电泳检测小反刍兽疫（PPR）病毒抗原。
J Hyg (Lond). 1984 Dec;93(3):579-86. doi: 10.1017/s0022172400065165.
2
The production of peste des petits ruminants hyperimmune sera in rabbits and their application in virus diagnosis.小反刍兽疫高免血清在兔体内的制备及其在病毒诊断中的应用。
Zentralbl Veterinarmed B. 1990 Jul;37(5):345-52. doi: 10.1111/j.1439-0450.1990.tb01068.x.
3
The isolation of peste des petits ruminants virus from Nigerian sheep and goats.从尼日利亚绵羊和山羊中分离小反刍兽疫病毒。
Res Vet Sci. 1979 Jan;26(1):94-6.
4
Improvement and development of rapid chromatographic strip-tests for the diagnosis of rinderpest and peste des petits ruminants viruses.口蹄疫和小反刍兽疫病毒快速层析检测试纸条的改进与研制。
J Virol Methods. 2011 Jun;174(1-2):42-6. doi: 10.1016/j.jviromet.2011.03.016. Epub 2011 Mar 17.
5
Pathogenicity of peste des petits ruminants virus isolated from Egyptian goats in Egypt.
Arch Exp Veterinarmed. 1990;44(5):789-92.
6
Specific detection of peste des petits ruminants virus antibodies in sheep and goat sera by the luciferase immunoprecipitation system.利用荧光素酶免疫沉淀系统特异性检测绵羊和山羊血清中的小反刍兽疫病毒抗体
J Virol Methods. 2016 Jan;227:40-6. doi: 10.1016/j.jviromet.2015.10.008. Epub 2015 Oct 23.
7
The isolation of adenoviruses from goats affected with peste des petits ruminants in Nigeria.
Res Vet Sci. 1977 Nov;23(3):331-5.
8
Development and distribution of rinderpest virus antigen in experimentally infected goats.牛瘟病毒抗原在实验感染山羊体内的发育与分布
Vet Rec. 1988 Aug 20;123(8):199-200. doi: 10.1136/vr.123.8.199.
9
Interference in the vaccination of cattle against rinderpest virus by antibodies against peste des petits ruminants (PPR) virus.抗小反刍兽疫（PPR）病毒抗体对牛瘟病毒疫苗接种的干扰。
Vaccine. 2006 Jul 17;24(29-30):5679-83. doi: 10.1016/j.vaccine.2006.04.052. Epub 2006 May 15.
10
Experimental peste des petits ruminants (goat plague) in goats and sheep.山羊和绵羊的实验性小反刍兽疫（羊瘟）
Can J Vet Res. 1988 Jan;52(1):46-52.
引用本文的文献
1
Paradigm shift in the diagnosis of peste des petits ruminants: scoping review.小反刍兽疫诊断范式的转变：范围综述。
Acta Vet Scand. 2020 Jan 29;62(1):7. doi: 10.1186/s13028-020-0505-x.
2
Peste des petits ruminants in Africa: Meta-analysis of the virus isolation in molecular epidemiology studies.非洲小反刍兽疫：分子流行病学研究中病毒分离的荟萃分析
Onderstepoort J Vet Res. 2019 Mar 26;86(1):e1-e15. doi: 10.4102/ojvr.v86i1.1677.
3
Diagnosis and control of peste des petits ruminants: a comprehensive review.小反刍兽疫的诊断与防控：综述
Virusdisease. 2014 Jan;25(1):39-56. doi: 10.1007/s13337-013-0188-2. Epub 2013 Nov 27.
4
A simple and rapid colloidal gold-based immunochromatogarpic strip test for detection of FMDV serotype A.一种用于检测口蹄疫病毒 A 型的简单快速胶体金免疫层析试纸条检测方法。
Virol Sin. 2011 Feb;26(1):30-9. doi: 10.1007/s12250-011-3166-5. Epub 2011 Feb 18.
5
Antibody seroprevalences against Peste des Petits Ruminants (PPR) virus in sheep and goats in Sudan.苏丹绵羊和山羊中抗小反刍兽疫（PPR）病毒的抗体血清阳性率。
Trop Anim Health Prod. 2009 Oct;41(7):1449-53. doi: 10.1007/s11250-009-9333-8.
6
Comparative efficacy of standard AGID and precipitinogen inhibition test with monoclonal antibodies based competitive ELISA for the serology of Peste des Petits Ruminants in sheep and goats.标准琼脂免疫扩散试验（AGID）和沉淀原抑制试验与基于单克隆抗体的竞争酶联免疫吸附测定法（ELISA）在绵羊和山羊小反刍兽疫血清学检测中的比较效力
Trop Anim Health Prod. 2009 Mar;41(3):413-20. doi: 10.1007/s11250-008-9205-7. Epub 2008 Jul 13.
7
Development of an indirect ELISA for the detection of antibodies against Peste-des-petits-ruminants virus in small ruminants.用于检测小反刍兽疫病毒抗体的间接酶联免疫吸附测定法在小反刍动物中的开发。
Vet Res Commun. 2007 Apr;31(3):355-64. doi: 10.1007/s11259-006-3442-x. Epub 2006 Dec 23.
8
One-step multiplex RT-PCR assay for the detection of peste des petits ruminants virus in clinical samples.用于检测临床样本中小反刍兽疫病毒的一步多重逆转录聚合酶链反应检测法。
Vet Res Commun. 2006 Aug;30(6):655-66. doi: 10.1007/s11259-006-3331-3.
9
Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.基于单克隆抗体的夹心酶联免疫吸附测定法与病毒分离法在山羊组织和分泌物中检测小反刍兽疫病毒的比较
J Clin Microbiol. 1994 May;32(5):1349-53. doi: 10.1128/jcm.32.5.1349-1353.1994.
10
Dot enzyme immunoassay for visual detection of peste-des-petits-ruminants virus antigen from infected caprine tissues.用于从感染山羊组织中目视检测小反刍兽疫病毒抗原的斑点酶免疫测定法。
J Clin Microbiol. 1989 Sep;27(9):2096-9. doi: 10.1128/jcm.27.9.2096-2099.1989.
本文引用的文献
1
Serodiagnosis of Dermatophilus congolensis infection by counterimmunoelectrophoresis.用对流免疫电泳法对刚果嗜皮菌感染进行血清学诊断。
Res Vet Sci. 1982 Nov;33(3):265-9.
2
Rapid detection of rinderpest virus antigens by counter-immunoelectrophoresis.应用对流免疫电泳法快速检测牛瘟病毒抗原
Trop Anim Health Prod. 1980 Nov;12(4):209-16. doi: 10.1007/BF02236618.
3
Detection of rinderpest antigen by agar gel diffusion and counter-immunoelectrophoresis.用琼脂凝胶扩散法和对流免疫电泳法检测牛瘟抗原。
Trop Anim Health Prod. 1983 May;15(2):83-5. doi: 10.1007/BF02239800.
4
Etiology of the stomatitis pneumoenteritis complex in Nigerian dwarf goats.尼日利亚矮山羊口炎肺炎肠炎综合征的病因
Can J Comp Med. 1976 Jul;40(3):276-84.
5
The isolation of peste des petits ruminants virus from Nigerian sheep and goats.从尼日利亚绵羊和山羊中分离小反刍兽疫病毒。
Res Vet Sci. 1979 Jan;26(1):94-6.
6
Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus.小反刍兽疫病毒被归类为麻疹病毒属的第四个成员。
Intervirology. 1979;11(5):268-74. doi: 10.1159/000149044.
7
Confirmation of rinderpest from samples of affected gums.从受感染牙龈样本中确诊牛瘟。
Trop Anim Health Prod. 1977 Nov;9(4):232. doi: 10.1007/BF02240344.
Zotero 插件