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用琼脂凝胶沉淀试验和对流免疫电泳检测小反刍兽疫(PPR)病毒抗原。

The detection of peste des petits ruminants (PPR) virus antigen by agar gel precipitation test and counter-immunoelectrophoresis.

作者信息

Obi T U, Patrick D

出版信息

J Hyg (Lond). 1984 Dec;93(3):579-86. doi: 10.1017/s0022172400065165.

DOI:10.1017/s0022172400065165
PMID:6512258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129473/
Abstract

The detectability of peste des petits ruminants (PPR) viral antigen in both ante-mortem secretions and necropsy samples from experimentally infected goats was investigated by both the agar gel precipitation test (AGPT) and counter-immunoelectrophoresis (CIE). Viral antigen was detected from 42.6% of the samples tested by the AGPT and 80.3% by CIE. The detection of viral antigen in a high proportion of the ocular and nasal secretions as well as the faeces and buccal scrapings, particularly from those collected within seven days of the onset of fever, by both techniques, would seem to obviate the need for lymph node biopsies or post-mortem samples in order to make a diagnosis of PPRV infection.

摘要

通过琼脂凝胶沉淀试验(AGPT)和对流免疫电泳(CIE),研究了实验感染山羊生前分泌物和尸检样本中绵羊痘和山羊痘(PPR)病毒抗原的可检测性。AGPT检测的样本中有42.6%检测到病毒抗原,CIE检测的样本中有80.3%检测到病毒抗原。两种技术在高比例的眼鼻分泌物、粪便和颊部刮片中检测到病毒抗原,特别是在发热开始后7天内采集的样本中,这似乎消除了为诊断PPRV感染而进行淋巴结活检或尸检样本的必要性。

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本文引用的文献

1
Serodiagnosis of Dermatophilus congolensis infection by counterimmunoelectrophoresis.用对流免疫电泳法对刚果嗜皮菌感染进行血清学诊断。
Res Vet Sci. 1982 Nov;33(3):265-9.
2
Rapid detection of rinderpest virus antigens by counter-immunoelectrophoresis.应用对流免疫电泳法快速检测牛瘟病毒抗原
Trop Anim Health Prod. 1980 Nov;12(4):209-16. doi: 10.1007/BF02236618.
3
Detection of rinderpest antigen by agar gel diffusion and counter-immunoelectrophoresis.用琼脂凝胶扩散法和对流免疫电泳法检测牛瘟抗原。
Trop Anim Health Prod. 1983 May;15(2):83-5. doi: 10.1007/BF02239800.
4
Etiology of the stomatitis pneumoenteritis complex in Nigerian dwarf goats.尼日利亚矮山羊口炎肺炎肠炎综合征的病因
Can J Comp Med. 1976 Jul;40(3):276-84.
5
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6
Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus.小反刍兽疫病毒被归类为麻疹病毒属的第四个成员。
Intervirology. 1979;11(5):268-74. doi: 10.1159/000149044.
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Confirmation of rinderpest from samples of affected gums.从受感染牙龈样本中确诊牛瘟。
Trop Anim Health Prod. 1977 Nov;9(4):232. doi: 10.1007/BF02240344.