Division of Medical Sciences, Island Medical Program, University of Victoria, Victoria, British Columbia, Canada.
Cell Commun Signal. 2013 Aug 21;11:62. doi: 10.1186/1478-811X-11-62.
Pannexins (Panxs) are relatively newly discovered large-pore ion and metabolite permeable channels. Although no proteomics-based interactome has yet been published, Panx1 has been demonstrated to interact with actin in an ectopic expression system. This interaction affects both Panx1 plasma membrane stability as well as cytoskeletal remodelling. The current study builds on our recent discovery of Panx1 expression in ventricular zone (VZ) neural stem and progenitor cells (NSC/NPCs), and on the demonstrated interaction of Panx1 with the cytoskeleton.
Here we demonstrate that Panx1 also plays roles in two additional cell behaviours associated with neurogenesis, including cell migration and neurite extension. Furthermore, we confirm an endogenous interaction between actin and Panx1, and identify a new interaction with actin-related protein 3, an actin cytoskeleton-modulating protein.
This study further establishes the importance of Panx1 in the cell biology of NSC/NPCs and strengthens and expands our knowledge of Panx1 interactions with the cytoskeleton.
连接蛋白（Panx）是新近发现的一种具有大孔离子和代谢物通透性的通道。尽管尚未发表基于蛋白质组学的相互作用组，但已经证明 Panx1 在异位表达系统中与肌动蛋白相互作用。这种相互作用不仅影响 Panx1 质膜的稳定性，还影响细胞骨架重塑。本研究是基于我们最近在室管膜区（VZ）神经干细胞和祖细胞（NSC/NPC）中发现 Panx1 的表达以及 Panx1 与细胞骨架的相互作用的基础上进行的。
我们在这里证明 Panx1 还在与神经发生相关的另外两种细胞行为中发挥作用，包括细胞迁移和神经突延伸。此外，我们证实了肌动蛋白与 Panx1 之间存在内源性相互作用，并鉴定了与肌动蛋白相关蛋白 3（一种调节细胞骨架的肌动蛋白细胞骨架蛋白）的新相互作用。
本研究进一步确立了 Panx1 在 NSC/NPC 细胞生物学中的重要性，并加强和扩展了我们对 Panx1 与细胞骨架相互作用的认识。
