Department of Anatomy, University of California San Francisco, San Francisco, California.
J Cell Biochem. 2014 Feb;115(2):209-16. doi: 10.1002/jcb.24658.
Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopes and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT). This amalgamation of techniques integrates 3D molecular localization data (CFT) with a high-resolution, 3D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near-native, cryopreserved state. Here we describe the current state of the art in correlative CFT-SXT, and discuss the future outlook for this method.
每种显微镜都仅限于成像细胞结构和/或分子组织的特定方面。然而，通过互补显微镜对标本进行成像并对数据进行关联，可以克服这一限制。虽然这不是一种新方法，但相关成像领域目前正受益于新显微镜技术的出现。在这里，我们描述了低温荧光断层扫描（CFT）与软 X 射线断层扫描（SXT）的关联。该技术的结合将 3D 分子定位数据（CFT）与细胞的高分辨率 3D 重建（SXT）相结合。细胞在近乎自然的冷冻保存状态下在两种模式下进行成像。在这里，我们描述了相关 CFT-SXT 的最新技术状态，并讨论了该方法的未来展望。
